Cellular and humoral immune aspects in mixed wife-husband leukocyte cultures in spontaneous abortions.

The possible role of feto--maternal cell--and humoral mediated immunological reactions, in mixed wife-husband one- and two-way stimulated leukocyte cultures (MWHLC) in autologous serum were investigated in a comparative study of 27 cases of normal and abnormal pregnancies (8 control and 19 cases of spontaneous abortions). Sera obtained from women after spontaneous abortions had a significantly higher stimulating effect on MWHLC than sera from women with normal pregnancies. In one-way stimulated MWHLC in cases of abortions, this effect was evident on maternal responding cells incubated with mitomycin treated paternal leukocytes.