McCracken A W, Mauney C U
J Clin Pathol. 1971 Oct;24(7):641-4. doi: 10.1136/jcp.24.7.641.
During a large epidemic of diphtheria, the technique of immunofluorescence was applied to specimens obtained from 310 patients, 77 of whom were diagnosed clinically as having the disease. The technique made use of commerical fluorescent antisera prepared against the somatic antigens of Corynebacterium diphtheriae. The results obtained by immunofluorescence of slides prepared directly from swabs were found to be unsatisfactory but when the swabs were subjected to prior incubation in a growth medium the results of immunofluorescence and bacterial culture agreed in 95% of specimens. Immunofluorescence applied to bacterial colonies obtained on primary isolation agreed completely with definitive bacterial identification. These two methods for the rapid identification of C. diphtheriae appeared to be as reliable as formal cultural and biochemical methods and could be usefully and economically applied to the examination of large numbers of clinical specimens during an epidemic.
在一次大规模白喉流行期间，免疫荧光技术被应用于从310名患者身上获取的标本，其中77名患者临床诊断患有该病。该技术使用了针对白喉棒状杆菌菌体抗原制备的商业荧光抗血清。直接从拭子制备的载玻片进行免疫荧光检测得到的结果并不理想，但当拭子先在生长培养基中孵育时，免疫荧光结果与细菌培养结果在95%的标本中一致。应用于初次分离得到的细菌菌落的免疫荧光与明确的细菌鉴定完全一致。这两种快速鉴定白喉棒状杆菌的方法似乎与传统的培养和生化方法一样可靠，并且在流行期间可有效且经济地应用于大量临床标本的检测。
