糖皮质激素对三种人胃癌细胞系中精氨酸酶产生的调控

Regulation of arginase production by glucocorticoid in three human gastric cancer cell lines.

作者信息

Wu C W, Wang S R, Chien S L, Yeh T H, Lian S L, Shimizu N, Lui W Y, P'eng F K, Chi C W

机构信息

Department of Surgery, Veterans General Hospital-Taipei, Taiwan, Republic of China.

出版信息

Life Sci. 1992;51(17):1355-61. doi: 10.1016/0024-3205(92)90635-3.

DOI:10.1016/0024-3205(92)90635-3

PMID:1406053

Abstract

Gastric cancer tissues have high levels of glucocorticoid receptors (GR) and arginase. To investigate the interrelation of glucocorticoid, GR and arginase, three human gastric cancer cell lines (AZ-521, NUGC-3, KATO-III) were treated with hydrocortisone in the presence or absence of a glucocorticoid antagonist RU38486. GR were found to be present in all three lines, and hydrocortisone significantly increased the production of total arginase in all 3 lines. The induction of arginase production by hydrocortisone was inhibited by RU38486. These findings suggest that the regulation of arginase production by hydrocortisone in gastric cancer cells is mediated through GR.

摘要

胃癌组织中糖皮质激素受体（GR）和精氨酸酶水平较高。为了研究糖皮质激素、GR和精氨酸酶之间的相互关系，在有或没有糖皮质激素拮抗剂RU38486存在的情况下，用氢化可的松处理三种人胃癌细胞系（AZ-521、NUGC-3、KATO-III）。发现所有三种细胞系中均存在GR，并且氢化可的松显著增加了所有三种细胞系中总精氨酸酶的产生。氢化可的松对精氨酸酶产生的诱导作用被RU38486抑制。这些发现表明，氢化可的松对胃癌细胞中精氨酸酶产生的调节是通过GR介导的。