Wu C W, Chang Y F, Yeh T H, Chang T J, Lui W Y, P'Eng F K, Chi C W
Department of Surgery, Veterans General Hospital-Taipei, Taiwan.
Dig Dis Sci. 1994 Dec;39(12):2689-94. doi: 10.1007/BF02087711.
Steroid hormone receptors in three human gastric adenocarcinoma cell lines and their transplanted tumors (except nontumorigenic KATO-III) in nude mice were determined by dextran-coated charcoal assay. Progesterone receptors (PgR) were found in all cell lines, transplanted NUGC-3, and AZ 521 tumors. Estrogen receptors (ER) were found in KATO-III cells, transplanted NUGC-3, and AZ 521 tumors, whereas glucocorticoid receptors (GR) were found only in NUGC-3 tumor and no androgen receptor was found in any cell lines or transplanted tumors. Since NUGC-3 cells had ER, PgR, and GR, it was used for the study of the effects of steroid hormones on growth. The results showed the cell cycle phase distributions and growth rate of transplanted tumors were similar in hormone-treated and nontreated groups. The persistent expression of PgR in gastric cancer cell lines and tumors, and the slight increase of tumor volumes in the progesterone-treated group suggests that progesterone and its receptors may be important in the pathogenesis of gastric cancer, but their biological function remains to be elucidated.
采用葡聚糖包被活性炭法测定了三种人胃癌细胞系及其在裸鼠体内移植瘤（非致瘤性的KATO-III除外）中的类固醇激素受体。在所有细胞系、移植的NUGC-3和AZ 521肿瘤中均发现了孕激素受体（PgR）。在KATO-III细胞、移植的NUGC-3和AZ 521肿瘤中发现了雌激素受体（ER），而糖皮质激素受体（GR）仅在NUGC-3肿瘤中发现，在任何细胞系或移植瘤中均未发现雄激素受体。由于NUGC-3细胞具有ER、PgR和GR，因此用于研究类固醇激素对生长的影响。结果显示，激素处理组和未处理组移植瘤的细胞周期阶段分布和生长速率相似。胃癌细胞系和肿瘤中PgR的持续表达以及孕激素处理组肿瘤体积的轻微增加表明，孕激素及其受体可能在胃癌发病机制中起重要作用，但其生物学功能仍有待阐明。
