Kent A, Redmond E M, Keenan A K
Department of Pharmacology, University College, Belfield, Dublin, Ireland.
Life Sci. 1992;51(18):1439-44. doi: 10.1016/0024-3205(92)90538-z.
The possible inhibition of adenylyl cyclase activity by atrial peptides selective for the ANF-C receptor was investigated in bovine pulmonary artery endothelial cells. In these cells isoprenaline, guanine nucleotide and forskolin dose-dependently increased activity over basal levels. In the presence of rANF(99-126), these dose-dependent increases were not reduced, nor were they affected by the ANF-C receptor selective analogue C-ANF(102-121). Furthermore, the selective analogues rANF(103-123) and des[Cys105,Cys121]rANF104-126 had no effect on basal or stimulated adenylyl cyclase activity. It can be concluded that ANF-C receptors are not linked to inhibition of adenylyl cyclase in these cells.
在牛肺动脉内皮细胞中研究了对ANF-C受体具有选择性的心房肽对腺苷酸环化酶活性的可能抑制作用。在这些细胞中，异丙肾上腺素、鸟嘌呤核苷酸和福斯高林可使活性比基础水平呈剂量依赖性增加。在存在rANF(99 - 126)的情况下，这些剂量依赖性增加并未降低，也不受ANF-C受体选择性类似物C-ANF(102 - 121)的影响。此外，选择性类似物rANF(103 - 123)和des[Cys105,Cys121]rANF104 - 126对基础或刺激的腺苷酸环化酶活性均无影响。可以得出结论，在这些细胞中，ANF-C受体与腺苷酸环化酶的抑制作用无关。
