提高了对来自大脑的磷脂的31P核磁共振谱的分辨率。
Improved resolution of 31P nuclear magnetic resonance spectra of phospholipids from brain.
作者信息
Capuani G, Aureli T, Miccheli A, Di Cocco M E, Ramacci M T, Delfini M
机构信息
Institute for Research on Senescence, Pomezia, Rome.
出版信息
Lipids. 1992 May;27(5):389-91. doi: 10.1007/BF02536154.
PMID:1406067
Abstract
A method is described wherein the resolution of 31P nuclear magnetic resonance spectra of the lipophilic fraction from a Bligh-Dyer extract of rat brain can be enhanced. The lipids are dispersed as micelles in aqueous solution with sodium deoxycholate, and spectral resolution is further optimized by adjusting the pH and by removing ions from the solution. The application of the method to the study of aging in rat brain serves as an example.
摘要
本文描述了一种能够增强大鼠脑布莱-戴尔提取物亲脂部分31P核磁共振谱分辨率的方法。脂质与脱氧胆酸钠以胶束形式分散于水溶液中，通过调节pH值和去除溶液中的离子进一步优化光谱分辨率。以该方法应用于大鼠脑衰老研究为例。
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