Trower M K, Lenstra R, Omer C, Buchholz S E, Sariaslani F S
Central Research and Development Department, E. I. du Pont de Nemours and Company, Inc., Experimental Station, Wilmington, Delaware 19880-0228.
Mol Microbiol. 1992 Aug;6(15):2125-34. doi: 10.1111/j.1365-2958.1992.tb01386.x.
Xenobiotic transformation by Streptomyces griseus (ATCC13273) is catalysed by a cytochrome P-450, designated cytochrome P-450soy. A DNA segment carrying the structural gene encoding P-450soy (soyC) was cloned using an oligonucleotide probe constructed from the protein sequence of a tryptic peptide. Following DNA sequencing the deduced amino acid sequence of P-450soy was compared with that for P-450cam, revealing conservation of important structural components including the haem pocket. Expression of the cloned soyC gene product was demonstrated in Streptomyces lividans by reduced CO:difference spectral analysis and Western blotting. Downstream of soyC, a gene encoding a putative [3Fe-4S] ferredoxin (soyB), named ferredoxinsoy, was identified.
