Sariaslani F S, McGee L R, Trower M K, Kitson F G
Central Research and Development Department, E. I. duPont de Nemours & Company, Wilmington, DE 19880-0228.
Biochem Biophys Res Commun. 1990 Jul 31;170(2):456-61. doi: 10.1016/0006-291x(90)92113-e.
Oxidation of (+) camphor by cytochrome P-450soy-enriched intact cells of Streptomyces griseus resulted in the formation of one major and several minor metabolites. The minor metabolites were identified as 3-endo-hydroxycamphor (2%), 5-endo-hydroxycamphor (7%), 5-exo-hydroxycamphor (9%), 2,5-diketobornane (2%), and camphorquinone (3%). The major metabolite was isolated and conclusively identified as 6-endo-hydroxycamphor (60%). When supplemented with NADPH, spinach ferredoxin:NADP oxidoreductase and spinach ferredoxin, homogeneous preparations of cytochrome P-450soy oxidized camphor to a mixture of 3-endo-, 5-endo-, 5-exo-and 6-endo-hydroxycamphor. The data presented indicates that cytochrome P-450soy resembles its mammalian counterparts in its lack of regio- and stereospecificity in camphor oxidation.
用富含细胞色素P - 450soy的灰色链霉菌完整细胞氧化（+）樟脑，产生了一种主要代谢物和几种次要代谢物。次要代谢物被鉴定为3 - 内型羟基樟脑（2%）、5 - 内型羟基樟脑（7%）、5 - 外型羟基樟脑（9%）、2,5 - 二酮冰片烷（2%）和樟脑醌（3%）。主要代谢物被分离出来并最终鉴定为6 - 内型羟基樟脑（60%）。当添加NADPH、菠菜铁氧还蛋白：NADP氧化还原酶和菠菜铁氧还蛋白时，细胞色素P - 450soy的纯制剂将樟脑氧化为3 - 内型、5 - 内型、5 - 外型和6 - 内型羟基樟脑的混合物。所呈现的数据表明，细胞色素P - 450soy在樟脑氧化中缺乏区域和立体特异性，这一点与它的哺乳动物对应物相似。
