Trower M K, Sariaslani F S, O'Keefe D P
E. I. du Pont de Nemours and Company, Central Research and Development Department, Wilmington, Delaware 19880-0228.
J Bacteriol. 1989 Apr;171(4):1781-7. doi: 10.1128/jb.171.4.1781-1787.1989.
A soybean flour-induced, soluble cytochrome P-450 (P-450soy) was purified 130-fold to homogeneity from Streptomyces griseus. Native cytochrome P-450soy is a single polypeptide, with a molecular weight of 47,500, in association with one ferriprotoporphyrin IX prosthetic group. Oxidized P-450soy exhibited visible absorption maxima at 394, 514, and 646 nm, characteristic of a high-spin cytochrome P-450. The CO-reduced difference spectrum of P-450soy had a Soret maximum at 448 nm. When reconstituted with spinach ferredoxin and spinach ferredoxin:NADP+ oxidoreductase, purified cytochrome P-450soy catalyzed the NADPH-dependent oxidation of the xenobiotic substrates precocene II and 7-ethoxycoumarin. In vitro proteolysis of cytochrome P-450soy generated a stable and catalytically active cytochrome P-450, designated P-450soy delta.
从灰色链霉菌中纯化出一种大豆粉诱导的可溶性细胞色素P-450（P-450soy），纯化倍数达130倍，达到同质。天然细胞色素P-450soy是一种单一多肽，分子量为47,500，与一个亚铁原卟啉IX辅基结合。氧化型P-450soy在394、514和646nm处呈现可见吸收最大值，这是高自旋细胞色素P-450的特征。P-450soy的CO还原差光谱在448nm处有一个Soret最大值。当与菠菜铁氧还蛋白和菠菜铁氧还蛋白：NADP +氧化还原酶重组时，纯化的细胞色素P-450soy催化异生物质底物早熟素II和7-乙氧基香豆素的NADPH依赖性氧化。细胞色素P-450soy的体外蛋白水解产生一种稳定且具有催化活性的细胞色素P-450，命名为P-450soyδ。
