Ouyang Jia, Yan Ming, Kong Dechong, Xu Lin
College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, PR China.
Biotechnol J. 2006 Nov;1(11):1266-74. doi: 10.1002/biot.200600103.
The complete protein pattern of cellulase and hemicellulase genes was studied through the Genome-wide analysis in Trichoderma reesei. The genome database revealed the presence of 39 ORFs encoding related proteins, including 32 enzymes with a catalysis domain related to cellulases and hemicellulases and 7 related proteins with a cellulose-binding module (CBM). Ten of these encoded yet undescribed enzymes, including six novel beta-glucosidases or xylosidases, two putative xylanases and two undescribed mannases. To better illustrate the relation of these 39 related proteins, four groups were created and analyzed by phylogenetic analysis: group A corresponding to xylanases, group B belonging to mannases and acting to degrade mannan; group C containing all known and putative cellulose-degrading proteins that have highly conserved CBMs; and group D containing beta-glucosidase and beta-xylosidase. Group D was the largest group, in which 8 beta-glucosidases appeared to be non-secreted proteins.
通过对里氏木霉的全基因组分析，研究了纤维素酶和半纤维素酶基因的完整蛋白质模式。基因组数据库显示存在39个编码相关蛋白质的开放阅读框（ORF），其中包括32种具有与纤维素酶和半纤维素酶相关催化结构域的酶，以及7种具有纤维素结合模块（CBM）的相关蛋白质。其中有10种编码尚未描述的酶，包括6种新型β-葡萄糖苷酶或木糖苷酶、2种假定的木聚糖酶和2种未描述的甘露聚糖酶。为了更好地阐明这39种相关蛋白质之间的关系，通过系统发育分析创建并分析了四组：A组对应木聚糖酶，B组属于甘露聚糖酶且作用于降解甘露聚糖；C组包含所有具有高度保守CBM的已知和假定的纤维素降解蛋白；D组包含β-葡萄糖苷酶和β-木糖苷酶。D组是最大的组，其中8种β-葡萄糖苷酶似乎是非分泌蛋白。
