LASZLO I
Br J Pharmacol Chemother. 1963 Aug;21(1):113-26. doi: 10.1111/j.1476-5381.1963.tb01507.x.
Several methods for removing interfering nucleotides, adenosine-5'-monophosphate and adenosine 5'-triphosphate from brain extracts have been studied. An enzymic method, using adenylic acid deaminase, has been found suitable. This deaminates adenosine monophosphate to 5'-inosinic acid, an inactive compound which does not influence the estimations of substance P. Owing to the adenosine triphosphatase content of the enzyme extract, adenosine triphosphate was also inactivated. For the estimation of adenosine monophosphate-deaminase activity, a simple colorimetric method is described which measures the ammonia liberated from adenosine monophosphate. Substance P in mouse brain extracts was estimated after treatment of the animals with various drugs, and after the enzymic removal of interfering nucleotides from the brain extracts. The drugs had no effect on the substance P content of mouse brain. The effect of drugs on the contractions of the guinea-pig ileum induced by substance P was also investigated, and the effect of drugs on the estimations of substance P in brain extracts is discussed.
已经研究了几种从脑提取物中去除干扰性核苷酸、5'-单磷酸腺苷和5'-三磷酸腺苷的方法。已发现一种使用腺苷酸脱氨酶的酶法是合适的。该酶将单磷酸腺苷脱氨生成5'-肌苷酸，这是一种不影响P物质测定的无活性化合物。由于酶提取物中含有三磷酸腺苷酶，三磷酸腺苷也被灭活。为了测定腺苷酸脱氨酶的活性，描述了一种简单的比色法，该方法测量从单磷酸腺苷释放的氨。在用各种药物处理动物后，以及在从脑提取物中酶法去除干扰性核苷酸后，对小鼠脑提取物中的P物质进行了测定。这些药物对小鼠脑内P物质含量没有影响。还研究了药物对P物质诱导的豚鼠回肠收缩的影响，并讨论了药物对脑提取物中P物质测定的影响。
