脱氧核糖核酸在枯草芽孢杆菌转化系统中的掺入
INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.
作者信息
YOUNG F E, SPIZIZEN J
出版信息
J Bacteriol. 1963 Sep;86(3):392-400. doi: 10.1128/jb.86.3.392-400.1963.
Abstract
Young, F. E. (Western Reserve University Cleveland, Ohio) and John Spizizen. Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system. J. Bacteriol. 86:392-400. 1963.-The optimal conditions for the incorporation of deoxyribonucleic acid (DNA) were studied. In competent cells, the irreversible binding of DNA was influenced by temperature, hydrogen ion concentration, and aeration. Divalent cations, such as barium, strontium, calcium, or magnesium, were required. Under suboptimal environmental conditions and with metabolic inhibitors, the process of transformation was decreased to a greater extent than was incorporation of DNA. Under conditions of phosphate depletion, the incorporation of P(32) increased. However, the frequency of transformation decreased. This inducible process was not related to competence.
摘要
扬，F.E.（俄亥俄州克利夫兰市西储大学）和约翰·斯皮齐曾。枯草芽孢杆菌转化系统中脱氧核糖核酸的掺入。《细菌学杂志》86:392 - 400。1963年。——研究了脱氧核糖核酸（DNA）掺入的最佳条件。在感受态细胞中，DNA的不可逆结合受温度、氢离子浓度和通气的影响。需要二价阳离子，如钡、锶、钙或镁。在次优环境条件下以及使用代谢抑制剂时，转化过程的降低程度比DNA掺入的降低程度更大。在磷酸盐耗尽的条件下，P(32)的掺入增加。然而，转化频率降低。这个诱导过程与感受态无关。
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