LEONARD C G, MATTHEIS D K, MATTHEIS M J, HOUSEWRIGHT R D
J Bacteriol. 1964 Jul;88(1):220-5. doi: 10.1128/jb.88.1.220-225.1964.
Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright. Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bacteriol. 88:220-225. 1964.-Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimal growth conditions for a serine-deficient mutant transformation frequencies of 0.1% were obtained. A medium for transformation of competent cells in liquid suspensions is described. In addition, this report presents evidence for the transformation of three nonencapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).
伦纳德、C. 戈麦斯（美国陆军生物实验室，马里兰州弗雷德里克市德特里克堡）、D. K. 马西斯、M. J. 马西斯和R. D. 豪斯赖特。地衣芽孢杆菌向原养型的转化及聚谷氨酸的合成。《细菌学杂志》88:220 - 225。1964年。——研究了地衣芽孢杆菌14种营养缺陷型菌株转化为原养型所需的生长条件。讨论了每个营养缺陷型菌株对可转化细胞发育的不同生长需求这一意外发现。在丝氨酸缺陷型突变体的最佳生长条件下，获得了0.1%的转化频率。描述了一种用于液体悬浮液中感受态细胞转化的培养基。此外，本报告提供了地衣芽孢杆菌三个非荚膜突变体转化为合成聚谷氨酸（荚膜物质）能力的证据。
