EDWARDS M R, STEVENS R W
J Bacteriol. 1963 Sep;86(3):414-28. doi: 10.1128/jb.86.3.414-428.1963.
Edwards, Mercedes R. (New York State Department of Health, Albany) and Roy W. Stevens. Fine structure of Listeria monocytogenes. J. Bacteriol. 86:414-428. 1963.-Cells of Listeria monocytogenes, at different stages of growth, were fixed with osmium tetroxide and treated with uranyl acetate. The material was dehydrated in alcohol, embedded in prepolymerized methacrylate, and studied in thin sections. In most of the micrographs, the plasmalemma (or plasma membrane) showed a pattern of three dense lines, each ca. 25 A thick, alternating with two light zones, each ca. 30 A thick. The outer light zone was regularly bridged by strands of dense material, and the inner one was not. The dense line at the edge of the cytoplasm was not always discernible because of its similarity in density with the ground cytoplasm, although it could be easily demonstrated in lysed cells and in protoplasts. The latter were found to be limited by a pair of dense lines, each ca. 25 A thick, bounding a light core ca. 30 A thick. This structure corresponds to a "unit" membrane, but it represents only a part of the plasmalemma of the intact cell; it was therefore interpreted as being more complex than a single unit membrane. Intracytoplasmic membranes of various configurations were clearly shown to be extensions of the plasmalemma. They may branch repeatedly and anastomose to form a complicated honeycomb-like organelle or organelles of different appearances, sometimes lamellate. The lamellar bodies are envisioned as resulting from spiraled ingrowths. The various kinds of ingrowths of the plasmalemma were designated "plasmalemmosomes" to indicate their origin; however, some of these organelles in Listeria were similar to those described in different bacteria by other authors. Plasmalemmosomes have been found in both aerobically and anaerobically grown cells. Another outstanding feature in many micrographs was the nucleoid, which contains dense fibrils measuring 25 to 50 A in diameter. These fibrils frequently appeared to be coiled and were of the order of magnitude ascribed to deoxyribonucleic acid molecules.
爱德华兹，梅赛德斯·R.（纽约州卫生部，奥尔巴尼）和罗伊·W.史蒂文斯。产单核细胞李斯特菌的精细结构。《细菌学杂志》86:414 - 428。1963年。——处于不同生长阶段的产单核细胞李斯特菌细胞用四氧化锇固定，并用醋酸铀处理。材料在酒精中脱水，包埋在预聚合的甲基丙烯酸酯中，并在薄切片中进行研究。在大多数显微照片中，质膜显示出三条致密线的模式，每条约25埃厚，与两个亮区交替，每个亮区约30埃厚。外部亮区由致密物质的股线规则地桥接，而内部亮区则没有。由于其密度与周围细胞质相似，细胞质边缘的致密线并不总是可辨别的，尽管它在裂解细胞和原生质体中很容易显示出来。发现后者由一对致密线限制，每条约25埃厚，界定一个约30埃厚的亮核。这种结构对应于一个“单位”膜，但它仅代表完整细胞质膜的一部分；因此，它被解释为比单个单位膜更复杂。各种构型的胞内膜被清楚地显示为质膜的延伸。它们可能反复分支并吻合，形成一个复杂的蜂窝状细胞器或不同外观的细胞器，有时呈层状。层状体被设想为由螺旋状向内生长形成。质膜的各种向内生长被称为“质膜体”以表明它们的起源；然而，李斯特菌中的一些这些细胞器与其他作者在不同细菌中描述的那些相似。质膜体在需氧和厌氧生长的细胞中都已被发现。许多显微照片中的另一个突出特征是类核，它包含直径为25至50埃的致密纤维。这些纤维经常看起来是盘绕的，并且与归属于脱氧核糖核酸分子的数量级相同。
