HOFFMAN H, FRANK M E
J Bacteriol. 1965 Jan;89(1):212-6. doi: 10.1128/jb.89.1.212-216.1965.
Hoffman, Heiner (New York University, New York, N.Y.), and Michael E. Frank. Time-lapse photomicrography of cell growth and division in Escherichia coli. J. Bacteriol. 89:212-216. 1965.-Photomicrographs at 15-sec intervals of cells growing at 37 C disclosed that in a cell with a generation time of 21.0 min the processes of furrowing, cross-wall formation, and cell separation are completed within 2.5 min after the division furrow first becomes clearly visible. Among a large number of cultivations examined, only a few cells late in one microculture at 43.5 C failed to separate once the cross wall was completed. Measurements of cell lengths during a 5-min period, extending from just before to just after division, showed that elongation of the cell is a discontinuous process, although the growth rate over the 5-min period is exponential. At the time of cell division, it appears that the synthesis of cell-wall material is diverted entirely into formation of the cross wall.
霍夫曼，海纳（纽约大学，纽约州纽约市），以及迈克尔·E·弗兰克。大肠杆菌细胞生长与分裂的延时显微摄影。《细菌学杂志》89:212 - 216。1965年。——对在37℃下生长的细胞每隔15秒拍摄的显微照片显示，在一个世代时间为21.0分钟的细胞中，沟裂、横壁形成和细胞分离过程在分裂沟首次清晰可见后的2.5分钟内完成。在大量检查的培养物中，在43.5℃下的一个微培养物后期只有少数细胞在横壁完成后未能分离。从刚好在分裂前到刚好在分裂后延伸的5分钟内对细胞长度的测量表明，细胞伸长是一个不连续的过程，尽管在这5分钟内的生长速率是指数性的。在细胞分裂时，细胞壁物质的合成似乎完全转向横壁的形成。
