鸡胚在维持和恢复淋病奈瑟菌毒力中的应用。
USE OF THE CHICK EMBRYO IN MAINTAINING AND RESTORING VIRULENCE OF NEISSERIA GONORRHOEAE.
作者信息
WALSH M J, BROWN B C, BROWN L, PIRKLE C I
出版信息
J Bacteriol. 1963 Sep;86(3):478-81. doi: 10.1128/jb.86.3.478-481.1963.
Walsh, Martin J. (Communicable Disease Center, Atlanta, Ga.), Bobby C. Brown, Leonard Brown, and Carl I. Pirkle. Use of the chick embryo in maintaining and restoring virulence of Neisseria gonorrhoeae. J. Bacteriol. 86:478-481. 1963.-Data based on its capabilities of producing acute urethritis in human male volunteers showed that Neisseria gonorrhoeae rapidly decreased in virulence after repeated subculture on chocolate agar medium. Cultures of a particular strain (GCM13) in the allantoic cavity of a developing chick embryo maintained virulence after 82 successive transfers. Another strain (GCF62), after loss of virulence through repeated subculture on chocolate agar, was again able to produce infection after 15 consecutive transfers in chick embryo. Thus, chick embryo apparently had the capability not only to maintain the virulence factor but also to reconstitute virulence. It is postulated that chick embryo serves as a selective medium for the growth of virulent gonococci, permitting rapid multiplication of these organisms and suppressing growth of the nonvirulent organisms.
沃尔什，马丁·J.（佐治亚州亚特兰大传染病中心），博比·C.布朗、伦纳德·布朗和卡尔·I.皮尔克尔。鸡胚在维持和恢复淋病奈瑟菌毒力中的应用。《细菌学杂志》86:478 - 481。1963年。-基于其在人类男性志愿者中引发急性尿道炎能力的数据表明，淋病奈瑟菌在巧克力琼脂培养基上反复传代后毒力迅速下降。某一特定菌株（GCM13）在发育中的鸡胚尿囊腔中的培养物在连续传代82次后仍保持毒力。另一菌株（GCF62）在巧克力琼脂上反复传代失去毒力后，在鸡胚中连续传代15次后又能够引发感染。因此，鸡胚显然不仅有能力维持毒力因子，还能恢复毒力。据推测，鸡胚作为有毒力淋球菌生长的选择性培养基，能使这些微生物快速繁殖并抑制无毒力微生物的生长。
Zotero 插件