Gibbs D L, Roberts R B
J Exp Med. 1975 Jan 1;141(1):155-71. doi: 10.1084/jem.141.1.155.
Cultivation of Neisseria gonorrhoeae in the allantoic cavity of 10-day chick embryos ensured the following necessary properties for subsequent quantitive in vitro phagocytosis studies of viable gonococci: log phase of growth, resistance to the cidal effect of fresh human serum, maintenance of colonial type, and absence of clumping. Employing a modification of the Maaloe technique, phagocytosis of log-phase type 1 and 2 gonococci by human PMN leukocytes did not occur in the presence or absence of serum. These findings indicate that log-phase type 1 and 2 gonococci possess antiphagocytic surface factors Stationary-phase organisms of the same colonial type were ingested and rapidly killed by human PMN leukocytes under similar experimental conditions, thus emphasizing the necessity to employ log-phase gonococci in the study of phagocytosis and antiphagocytic surface factors. Log-phase type 4 gonococci were ingested and rapidly killed by human PMN leukocytes in the presence of fresh human serum but not heat-inactivated serum or in the absence of serum. Morphologic studies demonstrated that log-phase viable gonococci attach to the surface membrane of human PMN leukocytes. Interiorization of avirulent but not virulent organisms was observed in the presence of fresh human serum. Gonococci-human PMN leukocyte interactions thus provide a model for the investigation of the nonimmunologic and immunologic parameters associated with the attachment and ingestion stages of phagocytosis.
在10日龄鸡胚的尿囊腔中培养淋病奈瑟菌，为后续对活淋球菌进行定量体外吞噬研究确保了以下必要特性：生长对数期、对新鲜人血清的杀菌作用具有抗性、菌落类型得以维持以及无凝集现象。采用对马尔勒技术的一种改良方法，在有或无血清存在的情况下，人PMN白细胞均未对对数期1型和2型淋球菌进行吞噬。这些发现表明，对数期1型和2型淋球菌具有抗吞噬表面因子。在类似实验条件下，相同菌落类型的稳定期菌体被人PMN白细胞摄取并迅速杀灭，这从而强调了在吞噬作用和抗吞噬表面因子研究中使用对数期淋球菌的必要性。在新鲜人血清存在的情况下，对数期4型淋球菌被人PMN白细胞摄取并迅速杀灭，但在热灭活血清存在的情况下或无血清时则不然。形态学研究表明，对数期活淋球菌附着于人PMN白细胞的表面膜。在新鲜人血清存在的情况下，观察到无毒力但非有毒力的菌体被内化。因此，淋球菌与人PMN白细胞的相互作用为研究与吞噬作用的附着和摄取阶段相关的非免疫和免疫参数提供了一个模型。
