脱硫脱硫弧菌的无机焦磷酸酶
INORGANIC PYROPHOSPHATASE OF DESULFOVIBRIO DESULFURICANS.
作者信息
AKAGI J M, CAMPBELL L L
出版信息
J Bacteriol. 1963 Sep;86(3):563-8. doi: 10.1128/jb.86.3.563-568.1963.
Akagi, J. M. (University of Illinois, Urbana) and L. Leon Campbell. Inorganic pyrophosphatase of Desulfovibrio desulfuricans. J. Bacteriol. 86:563-568. 1963.-The inorganic pyrophosphatase of Desulfovibrio desulfuricans was purified 136-fold by (NH(4))(2)SO(4) and ethanol fractionation and diethylaminoethyl cellulose chromatography. Mg(++) or Mn(++) was required for optimal activity; Co(++) was only 65% as effective as Mg(++). The optimal ratio of Mg(++) to pyrophosphate was 1.0 at pH 8.0. The K(s) for the pyrophosphatase was found to be in the region of 1.9 x 10(-3)m. Sulfhydryl inhibitors and sodium fluoride had no effect on enzyme activity at a concentration of 10(-3)m. The purified enzyme did not hydrolyze adenosine triphosphate, glycerol phosphate, diphenyl phosphate, or p-nitrophenyl phosphate. Thermal stability studies showed that the enzyme is rapidly inactivated at temperatures above 40 C.
赤木，J.M.（伊利诺伊大学厄巴纳分校）和L.利昂·坎贝尔。脱硫脱硫弧菌的无机焦磷酸酶。《细菌学杂志》86:563 - 568。1963年。——脱硫脱硫弧菌的无机焦磷酸酶通过硫酸铵和乙醇分级分离以及二乙氨基乙基纤维素色谱法纯化了136倍。最佳活性需要镁离子（Mg++）或锰离子（Mn++）；钴离子（Co++）的效果仅为镁离子的65%。在pH 8.0时，镁离子与焦磷酸的最佳比例为1.0。发现焦磷酸酶的米氏常数（K(s)）在1.9×10^(-3)摩尔的范围内。巯基抑制剂和氟化钠在10^(-3)摩尔的浓度下对酶活性没有影响。纯化后的酶不水解三磷酸腺苷、甘油磷酸、二苯磷酸或对硝基苯磷酸。热稳定性研究表明，该酶在温度高于40℃时会迅速失活。
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