Drake H L, Akagi J M
J Bacteriol. 1978 Dec;136(3):916-23. doi: 10.1128/jb.136.3.916-923.1978.
The reduction of bisulfite by Desulfovibrio vulgaris was investigated. Crude extracts reduced bisulfite to sulfide without the formation (detection) of any intermediates such as trithionate or thiosulfate. When the particulate fractions was removed from crude extracts by high-speed centrifugation, the soluble supernatant fraction reduced bisulfite sequentially to trithionate, thiosulfate, and sulfide. Addition of particles or purified membranes to the soluble fraction restored the original activity demonstrated by crude extracts, i.e., reduction of bisulfite to sulfide without the formation of trithionate and/or thiosulfate. By using antiserum directed against bisulfite reductase, the reduction of bisulfite by crude extracts was inhibited. This finding, in addition to several recycling studies of thiosulfate reduction, provided evidence that bisulfite reduction by D. vulgaris operated through the pathway involving trithionate and thiosulfate as intermediates. The role of membranes in this process is discussed.
研究了普通脱硫弧菌对亚硫酸氢盐的还原作用。粗提取物将亚硫酸氢盐还原为硫化物，未形成（检测到）任何中间产物，如连三硫酸盐或硫代硫酸盐。当通过高速离心从粗提取物中去除颗粒部分时，可溶性上清液部分将亚硫酸氢盐依次还原为连三硫酸盐、硫代硫酸盐和硫化物。向可溶性部分添加颗粒或纯化膜可恢复粗提取物所显示的原始活性，即亚硫酸氢盐还原为硫化物而不形成连三硫酸盐和/或硫代硫酸盐。通过使用针对亚硫酸氢盐还原酶的抗血清，粗提取物对亚硫酸氢盐的还原作用受到抑制。这一发现，除了几项硫代硫酸盐还原的循环研究外，还提供了证据表明普通脱硫弧菌对亚硫酸氢盐的还原作用是通过以连三硫酸盐和硫代硫酸盐为中间产物的途径进行的。讨论了膜在这一过程中的作用。
