KAWAKAMI M, OSAWA N, MITSUHASHI S
J Bacteriol. 1963 Oct;86(4):872-9. doi: 10.1128/jb.86.4.872-879.1963.
Kawakami, Masaya (Gunma University, Maebashi, Japan), Nobutaka Osawa, and Susumu Mitsuhashi. Experimental salmonellosis. III. New toxic fraction (L) obtained from Salmonella enteritidis and its immunological properties. J. Bacteriol. 86:872-879. 1963.-A method is described for the purification of the heat-labile toxins of a fully virulent strain, 116-54, of Salmonella enteritidis by ion-exchange chromatography. One component of the heat-labile toxin (L) was homogeneous, as evidenced by the results of the ultracentrifugal analysis and agar gel diffusion test. The mouse ld(50) was 1.3 mug, and chemical studies indicated that this toxin was a simple protein in nature. It was also evidenced by chemical and immunological tests that this toxin differs from the O antigen (lipopolysaccharide-protein complex).
川上正也（日本前桥市群马大学）、大泽信隆和三桥进。实验性沙门氏菌病。III. 从肠炎沙门氏菌中获得的新毒性组分（L）及其免疫学特性。《细菌学杂志》86:872 - 879。1963年。——描述了一种通过离子交换色谱法纯化肠炎沙门氏菌强毒株116 - 54的热不稳定毒素的方法。热不稳定毒素（L）的一个组分是均质的，超速离心分析和琼脂凝胶扩散试验的结果证明了这一点。小鼠半数致死量为1.3微克，化学研究表明这种毒素本质上是一种简单蛋白质。化学和免疫学试验也证明这种毒素与O抗原（脂多糖 - 蛋白质复合物）不同。
