EXPERIMENTAL SALMONELLOSIS. III. NEW TOXIC FRACTION (L) OBTAINED FROM SALMONELLA ENTERITIDIS AND ITS IMMUNOLOGICAL PROPERTIES.

Kawakami, Masaya (Gunma University, Maebashi, Japan), Nobutaka Osawa, and Susumu Mitsuhashi. Experimental salmonellosis. III. New toxic fraction (L) obtained from Salmonella enteritidis and its immunological properties. J. Bacteriol. 86:872-879. 1963.-A method is described for the purification of the heat-labile toxins of a fully virulent strain, 116-54, of Salmonella enteritidis by ion-exchange chromatography. One component of the heat-labile toxin (L) was homogeneous, as evidenced by the results of the ultracentrifugal analysis and agar gel diffusion test. The mouse ld(50) was 1.3 mug, and chemical studies indicated that this toxin was a simple protein in nature. It was also evidenced by chemical and immunological tests that this toxin differs from the O antigen (lipopolysaccharide-protein complex).