Milne M, Collins F M
J Bacteriol. 1966 Sep;92(3):543-8. doi: 10.1128/jb.92.3.543-548.1966.
Milne, Margaret (University of Adelaide, Adelaide, Australia), and F. M. Collins. Heat-labile antigens of Salmonella enteritidis. I. Extraction of antigens. J. Bacteriol. 92:543-548. 1966.-Salmonella enteritidis strains of high and low mouse virulence were grown in continuous culture. Cell walls were obtained from both strains by sonic disruption, and the washed walls were extracted at 4 C with sodium dodecyl sulfate or sodium deoxycholate. The extracts were eluted with a saline gradient from a diethylaminoethyl cellulose column, and the separated peaks were tested for precipitin activity against rabbit antisera prepared with heator ethyl alcohol-killed vaccines. The separated antigens reacted less intensely with the antiserum to heat-killed cells than they did to the antisera prepared against alcohol-killed vaccine. Little antigenic difference could be detected between the extracts prepared from the virulent and the avirulent organisms.
米尔恩，玛格丽特（澳大利亚阿德莱德阿德莱德大学）和F.M. 柯林斯。肠炎沙门氏菌的热不稳定抗原。I. 抗原的提取。《细菌学杂志》92:543 - 548。1966年。——高、低小鼠毒力的肠炎沙门氏菌菌株在连续培养中生长。通过超声破碎从两种菌株中获得细胞壁，洗涤后的细胞壁在4℃用十二烷基硫酸钠或脱氧胆酸钠提取。提取物用盐水梯度从二乙氨基乙基纤维素柱上洗脱，分离出的峰针对用加热或乙醇灭活疫苗制备的兔抗血清进行沉淀素活性测试。与抗热灭活细胞的抗血清相比，分离出的抗原与抗乙醇灭活疫苗制备的抗血清反应较弱。从有毒和无毒生物体制备的提取物之间几乎检测不到抗原差异。
