Balin A K, Goodman D B, Rasmussen H, Cristofalo V J
In Vitro. 1976 Oct;12(10):687-92. doi: 10.1007/BF02797472.
We have investigated that atmospheric stability in polystyrene and glass cell culture vessels by measuring the dissolved O2 and CO2 in the media of both seeded and unseeded culture vessels incubated at 37 degrees C. There was no diffusion of either O2 or CO2 through glass vessels. At low partial pressures of oxygen (PO2), oxygen diffused into the polystyrene flasks at a rate of 1 to 2 mmHg per 24 hr, and at high PO2, oxygen diffused slowly out of polystyrene flasks. CO2 diffused out of polystyrene flasks with a half-time of 260 hr resulting in a considerable elevation in pH. In seeded polystyrene flasks with the PO2 less than or equal to room air, cellular oxygen consumption was masked by the inward diffusion of oxygen. In addition, the fall in pH due to metabolic CO2 and organic acid production during cell growth in polystyrene flasks was buffered by the diffusion of CO2 out of the vessels.
我们通过测量在37摄氏度下孵育的接种和未接种培养容器培养基中的溶解氧和二氧化碳，研究了聚苯乙烯和玻璃细胞培养容器中的大气稳定性。氧气和二氧化碳都不会透过玻璃容器扩散。在低氧分压（PO2）下，氧气以每24小时1至2 mmHg的速率扩散到聚苯乙烯烧瓶中，而在高PO2下，氧气从聚苯乙烯烧瓶中缓慢扩散出来。二氧化碳以260小时的半衰期从聚苯乙烯烧瓶中扩散出来，导致pH值显著升高。在PO2小于或等于室内空气的接种聚苯乙烯烧瓶中，细胞的氧气消耗被氧气的向内扩散所掩盖。此外，聚苯乙烯烧瓶中细胞生长过程中由于代谢产生二氧化碳和有机酸导致的pH值下降，会被二氧化碳从容器中扩散出去所缓冲。
