Balin A K, Goodman D B, Rasmussen H, Cristofalo V J
J Cell Biol. 1977 Jul;74(1):58-67. doi: 10.1083/jcb.74.1.58.
Human diploid cells (WI-38) were serially subcultivated at partial pressures of oxygen (Po2) ranging from 5.6 mm Hg to 608 mm Hg. At a Po2 of 5.6 mm Hg, the number of doublings to phase out was less than that of control cells at a Po2 of 137 mm Hg. Cultures grown at Po2's of 24, 49, or 137 mm Hg grew at the same rate and phased out after a similar number of population doublings. Population lifespan was markedly shortened by chronic exposure to elevated Po2's, a phenomenon that was, in part, reversible. d-1-alpha-Tocopherol (10 microgram/ml or 100 microgram/ml) homogenized into the medium at each weekly subcultivation did not extend the lifespan of cells at reduced, ambient, or elevated oxygen tensions. These results indicate that neither oxygen toxicity nor free radical reactions play a significant role in limiting the lifespan of WI-38 cells grown in vitro under ambient oxygen tensions (Po2 137 mm Hg).
人二倍体细胞（WI - 38）在5.6毫米汞柱至608毫米汞柱的氧分压（Po2）下进行连续传代培养。在5.6毫米汞柱的Po2条件下，细胞停止增殖前的倍增次数少于在137毫米汞柱Po2条件下的对照细胞。在24、49或137毫米汞柱Po2条件下培养的细胞生长速率相同，且在经过相似数量的群体倍增后停止增殖。长期暴露于升高的Po2会显著缩短群体寿命，这一现象部分是可逆的。在每次每周传代时向培养基中加入d - 1 - α - 生育酚（10微克/毫升或100微克/毫升），在降低、正常或升高的氧张力条件下均未延长细胞寿命。这些结果表明，在正常氧张力（Po2 137毫米汞柱）下体外培养的WI - 38细胞，氧毒性和自由基反应在限制其寿命方面均未起重要作用。
