Tanswell A K, Joneja M G, Possmayer F, Harding P
In Vitro. 1984 Aug;20(8):635-41. doi: 10.1007/BF02619613.
Differentiation-arrested lung cell cultures were developed from fetal rats of various gestational ages. In contrast to previously published observations with cultures in a pO2 of approximately 142 mm Hg, cultures developed in a pO2 of approximately 30 mm Hg, close to the normal fetal arterial pO2, have improved plating efficiency and a slightly increased growth rate. They did not, however, show gestation-dependent increases of choline incorporation into phospholipids, nor did immature lung cell cultures respond to dexamethasone or triiodothyronine, singly or in combination, by increased choline incorporation into saturated lecithin. The incorporation of choline and glycerol into lipids suggested a mature rate of lipid synthesis by immature cultures at a pO2 approximately 30 mm Hg, despite preservation of an immature morphology. Electron microscope observations revealed no gross differences between immature cultures developed at either pO2. The cellular mechanisms underlying these differences are unclear but suggest that oxygen tension may significantly influence results obtained with in vitro studies of lipid synthesis by immature lung.
从不同胎龄的胎鼠中培养出分化停滞的肺细胞。与之前发表的在约142毫米汞柱的氧分压下进行培养的观察结果相反，在接近正常胎儿动脉氧分压的约30毫米汞柱的氧分压下培养的细胞，其接种效率有所提高，生长速率略有增加。然而，它们并未表现出胆碱掺入磷脂的量随胎龄增加，未成熟的肺细胞培养物也未对单独或联合使用的地塞米松或三碘甲状腺原氨酸作出反应，使胆碱掺入饱和卵磷脂的量增加。胆碱和甘油掺入脂质表明，尽管形态上仍未成熟，但在约30毫米汞柱的氧分压下，未成熟培养物的脂质合成速率已达到成熟水平。电子显微镜观察显示，在两种氧分压下培养的未成熟培养物之间没有明显差异。这些差异背后的细胞机制尚不清楚，但表明氧张力可能会显著影响未成熟肺脂质合成体外研究的结果。
