Cashman R E, Grammas P
Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA.
Mol Chem Neuropathol. 1995 Dec;26(3):247-58. doi: 10.1007/BF02815141.
The purpose of this study was to compare the effects of aging and Alzheimer disease (AD) on the important intracellular signaling enzyme cAMP-dependent protein kinase A (PKA) in cerebral microvessels. PKA activity and levels were measured in microvessels isolated from the brains of adult and aged rodents as well as from the cerebral cortices of AD and elderly control patients. The results showed that cerebral microvessels from aged rats have significantly (p < 0.01) higher PKA activity and levels when compared to cerebral microvessels from adult rats. In contrast, no significant difference was found between PKA activity or levels in cerebral microvessels from AD patients when compared to controls. These results indicate that in cerebral microvessels both PKA activity and levels increase with age but are unaffected by AD. The data suggest that protein phosphorylation in brain microvessels may be affected differentially by aging and dementia.
本研究的目的是比较衰老和阿尔茨海默病（AD）对脑微血管中重要的细胞内信号传导酶环磷酸腺苷依赖性蛋白激酶A（PKA）的影响。测定了从成年和老年啮齿动物大脑以及AD患者和老年对照患者大脑皮质分离的微血管中的PKA活性和水平。结果显示，与成年大鼠的脑微血管相比，老年大鼠的脑微血管具有显著更高（p < 0.01）的PKA活性和水平。相比之下，AD患者脑微血管中的PKA活性或水平与对照组相比未发现显著差异。这些结果表明，在脑微血管中，PKA活性和水平均随年龄增长而增加，但不受AD影响。数据表明，脑微血管中的蛋白质磷酸化可能受到衰老和痴呆的不同影响。
