通过冷冻断裂技术观察发现，有尾目和无尾目动物视网膜中的穆勒（神经胶质）细胞呈现出不同的形态。

Müller (glial) cells in the retina of urodeles and anurans reveal different morphology by means of freeze-fracturing.

作者信息

Wolburg H, Berg-von der Emde K, Naujoks-Manteuffel C

机构信息

Institute of Pathology, University of Tübingen, FRG.

出版信息

Neurosci Lett. 1992 Apr 13;138(1):89-92. doi: 10.1016/0304-3940(92)90479-q.

DOI:10.1016/0304-3940(92)90479-q

PMID:1407671

Abstract

Müller (glial) cells of the retina of various species of amphibia (urodeles and anurans) were investigated by means of the freeze-fracture technique. This was done because Müller cells in anamniotes were believed to differ from those in mammals in that they should lack the so-called orthogonal arrays of particles (OAP) which are a characteristic feature of Müller cells in mammalian retina. However, as we could demonstrate previously (Berg-von der Emde and Wolburg, Glia, 2 (1989) 458), fish retinal Müller cells also contain OAP in their membranes suggesting that OAP are a general marker of Müller cells in all vertebrates. As demonstrated in this study, Müller cells of urodeles (Batrachoseps attenuatus and Pleurodeles waltlii) are OAP-positive, whereas two anurans (Rana esculenta and Xenopus laevis) do not reveal any OAP in their Müller cell membranes. Under phylogenetic aspects, it appears very interesting that frogs are as yet the only vertebrate group that deviates from all other vertebrates in terms of Müller cell membrane morphology.

摘要

利用冷冻断裂技术对各种两栖动物（有尾目和无尾目）视网膜的米勒（神经胶质）细胞进行了研究。之所以这样做，是因为人们认为无羊膜动物的米勒细胞与哺乳动物的不同，在于它们应该缺乏所谓的颗粒正交排列（OAP），而这是哺乳动物视网膜中米勒细胞的一个特征。然而，正如我们之前所证明的（伯格 - 冯·德·埃姆德和沃尔堡，《神经胶质》，2(1989)458），鱼类视网膜的米勒细胞膜中也含有OAP，这表明OAP是所有脊椎动物中米勒细胞的一个普遍标志。正如本研究所示，有尾目动物（细趾蟾和虎纹钝口螈）的米勒细胞是OAP阳性的，而两种无尾目动物（食用蛙和非洲爪蟾）的米勒细胞膜中未显示出任何OAP。从系统发育的角度来看，青蛙是目前唯一在米勒细胞膜形态方面与所有其他脊椎动物不同的脊椎动物群体，这一点显得非常有趣。