口腔鳞状细胞癌中ras 21的免疫组织化学检测

Immunohistochemical detection of ras 21 in oral squamous cell carcinomas.

作者信息

Satoh M, Hatakeyama S, Sashima M, Suzuki A

机构信息

Department of Oral Pathology, School of Dentistry, Iwate Medical University, Japan.

出版信息

Oral Surg Oral Med Oral Pathol. 1992 Oct;74(4):469-72. doi: 10.1016/0030-4220(92)90298-5.

DOI:10.1016/0030-4220(92)90298-5

PMID:1408023

Abstract

The expression of the ras p21 in oral squamous cell carcinoma was examined immunohistochemically with the use of a monoclonal antibody NCC-RAS-001 with the avidin-biotin-peroxidase complex method. The expression of ras p21 product was detected in 65.7% (44 of 67 cases) of cancer patients. On the basis of the degree of histologic differentiation of the cancer cells, the incidence of ras p21 was found to be as follows: 63.4% (26 of 41 cases) were well differentiated; 86.7% (13 of 15 cases) were moderately differentiated; and 45.5% (5 of 11 cases) were poorly differentiated. The highest incidence was found in patients in their sixties--80.0% (16 of 20 cases). The incidence decreased to 40% in patients over 80 years of age. The incidence of ras p21 on the basis of location was as follows: 81.8% (9 of 11 cases) involved the buccal region, 70.6% (12 of 17 cases) were in the gingiva, and 55.0% (11 of 20 cases) were in the tongue.

摘要

采用抗生物素蛋白-生物素-过氧化物酶复合物法，使用单克隆抗体NCC-RAS-001对口腔鳞状细胞癌中ras p21的表达进行免疫组织化学检测。在65.7%（67例中的44例）癌症患者中检测到ras p21产物的表达。根据癌细胞的组织学分化程度，ras p21的发生率如下：高分化者为63.4%（41例中的26例）；中分化者为86.7%（15例中的13例）；低分化者为45.5%（11例中的5例）。六十多岁的患者发生率最高——80.0%（20例中的16例）。80岁以上患者的发生率降至40%。根据肿瘤部位，ras p21的发生率如下：颊部为81.8%（11例中的9例），牙龈为70.6%（17例中的12例），舌部为55.0%（20例中的11例）。