Yamada T, Takagi M, Shioda S
First Department of Oral Surgery, Tokyo, Japan.
Oral Surg Oral Med Oral Pathol. 1992 Jan;73(1):67-70. doi: 10.1016/0030-4220(92)90157-l.
Forty-seven cases of oral squamous cell carcinoma were examined immunohistochemically by the avidin-biotin peroxidase complex method with anti-epidermal growth factor (EGF) receptor mouse monoclonal antibody, and 24 cases (51%) were shown to have EGF receptor-positive cells. Correlation was strong between presence of EGF receptors and differentiation; the EGF receptor-positive cells were differentiated, whereas poorly differentiated tumors exhibited less intense staining. EGF receptor gene and c-erb B-1 by Southern blot analysis disclosed that one of 25 cases of squamous cell carcinoma exhibited a fourfold amplification of the gene.
用抗表皮生长因子（EGF）受体小鼠单克隆抗体，通过抗生物素蛋白-生物素过氧化物酶复合物法对47例口腔鳞状细胞癌进行免疫组织化学检查，结果显示24例（51%）有EGF受体阳性细胞。EGF受体的存在与分化之间有很强的相关性；EGF受体阳性细胞是分化型的，而低分化肿瘤的染色强度较弱。通过Southern印迹分析EGF受体基因和c-erb B-1，发现25例鳞状细胞癌中有1例该基因有4倍扩增。
