GERHARDT P, GALLUP D M
J Bacteriol. 1963 Nov;86(5):919-29. doi: 10.1128/jb.86.5.919-929.1963.
Gerhardt, Philipp (The University of Michigan, Ann Arbor), and D. M. Gallup. Dialysis flask for concentrated culture of microorganisms. J. Bacteriol 86:919-929. 1963.-A twin-chambered dialysis flask was designed with a supported membrane clamped between a reservoir of medium in the bottom and a small volume of culture above, the unit being mounted on a shaking machine to provide aeration and agitation. The performance of different dialysis membranes and membrane filters was compared in glucose-diffusion and bacterial-culture tests. Some of the variables in dialysis culture were assessed and the growth response was characterized, with Serratia marcescens as the test organism. The general usefulness and concentrating effect of dialysis culture were demonstrated in trials with 16 representative types of microorganisms. Dialysis culture was shown to be especially suitable for producing dense populations of cells or their macromolecular products in an environment free from complex medium constituents, for removing toxic products that limit growth or fermentation, and for supplying oxygen by diffusion without the damage from usual aeration procedures.
格哈特，菲利普（密歇根大学，安阿伯分校），以及D. M. 盖洛普。用于微生物浓缩培养的透析烧瓶。《细菌学杂志》86:919 - 929。1963年。——设计了一种双室透析烧瓶，在底部的培养基储存器和上方少量培养物之间夹有支撑膜，该装置安装在摇床上以提供通气和搅拌。在葡萄糖扩散和细菌培养试验中比较了不同透析膜和膜过滤器的性能。以粘质沙雷氏菌为受试生物，评估了透析培养中的一些变量并描述了生长反应。在对16种代表性微生物类型的试验中证明了透析培养的一般实用性和浓缩效果。结果表明，透析培养特别适合在无复杂培养基成分的环境中产生高密度的细胞或其大分子产物，去除限制生长或发酵的有毒产物，以及通过扩散供应氧气而不受常规通气程序的损害。
