Schaumburg F D, Kirsch E J
Appl Microbiol. 1966 Sep;14(5):761-6. doi: 10.1128/am.14.5.761-766.1966.
A compartmented, autoclavable culture vessel has been developed for the purpose of studying interactive associations of microorganisms which are essential to the anaerobic decomposition of sewage sludge. The unit employs sterile filter membranes to subdivide the interior culture space into individual compartments. Bacteria cultured in one compartment are denied access to adjacent compartments, even though rapid interchange of nutrients and metabolic waste products occurs throughout the unit. The obligate methane-forming anaerobe, Methanobacillus omelianskii has been successfully grown and concentrated in this system by use of a synthetic medium reduced with sodium sulfide. The feasibility of using this system to study microbial interactions was, in part, demonstrated by growing M. omelianskii in a thoroughly aerated medium which had been biologically reduced by Escherichia coli prior to inoculation with the anaerobe. Under this condition of simulated mixed culture growth, the cell yield of both microorganisms, as well as specific metabolic activities ascribed to each organism, was readily monitored.
为了研究对污水污泥厌氧分解至关重要的微生物之间的相互作用关系，已开发出一种可分隔、可高压灭菌的培养容器。该装置采用无菌滤膜将内部培养空间细分为各个隔室。在一个隔室中培养的细菌无法进入相邻隔室，尽管营养物质和代谢废物在整个装置中能快速交换。通过使用用硫化钠还原的合成培养基，专性产甲烷厌氧菌奥氏甲烷杆菌已在该系统中成功生长和富集。通过在彻底曝气的培养基中培养奥氏甲烷杆菌来证明使用该系统研究微生物相互作用的可行性，该培养基在接种厌氧菌之前已被大肠杆菌进行生物还原。在这种模拟混合培养生长的条件下，两种微生物的细胞产量以及归因于每种生物体的特定代谢活性都很容易监测。
