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Carbon-13 NMR in conformational analysis of nucleic acid fragments. Heteronuclear chemical shift correlation spectroscopy of RNA constituents.核酸片段构象分析中的碳-13核磁共振。RNA成分的异核化学位移相关光谱学。
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Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.采用氢检测二维异核相关光谱法对短DNA双链体的碳-13核磁共振谱进行归属。
Nucleic Acids Res. 1987 Jan 12;15(1):267-75. doi: 10.1093/nar/15.1.267.
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13C NMR of the bases of three DNA oligonucleotide duplexes: assignment methods and structural features.三种DNA寡核苷酸双链体碱基的13C核磁共振：归属方法与结构特征
Biochemistry. 1988 Oct 4;27(20):7902-9. doi: 10.1021/bi00420a048.
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Determination of the DNA sugar pucker using 13C NMR spectroscopy.使用碳-13核磁共振光谱法测定DNA糖的构象。
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A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.一种用于蛋白质1H、13C和15N谱顺序归属的新方法：异核三共振三维核磁共振光谱法。应用于钙调蛋白。
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天然丰度下含异核的DNA的异核核磁共振：耦合常数的便捷归属与提取

Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.

作者信息

Schmieder P, Ippel J H, van den Elst H, van der Marel G A, van Boom J H, Altona C, Kessler H

机构信息

Organisch-Chemisches Institut, TU München, Garching, Germany.

出版信息

Nucleic Acids Res. 1992 Sep 25;20(18):4747-51. doi: 10.1093/nar/20.18.4747.

DOI:10.1093/nar/20.18.4747
PMID:1408787
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334227/
Abstract

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.

摘要

两个异核质子-碳核磁共振实验应用于天然丰度碳的DNA八聚体d(TTGGCCAA)₂。它们实现了糖和碱基碳共振的完全归属。此外，还测定了几个异核耦合常数，包括质子-碳、质子-磷以及磷-碳耦合常数。这些信息可在合理的测量时间内获得，并为DNA结构的详细描述提供了有价值的信息。