天然丰度下含异核的DNA的异核核磁共振：耦合常数的便捷归属与提取
Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.
作者信息
Schmieder P, Ippel J H, van den Elst H, van der Marel G A, van Boom J H, Altona C, Kessler H
机构信息
Organisch-Chemisches Institut, TU München, Garching, Germany.
出版信息
Nucleic Acids Res. 1992 Sep 25;20(18):4747-51. doi: 10.1093/nar/20.18.4747.
Abstract
Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.
摘要
两个异核质子-碳核磁共振实验应用于天然丰度碳的DNA八聚体d(TTGGCCAA)₂。它们实现了糖和碱基碳共振的完全归属。此外，还测定了几个异核耦合常数，包括质子-碳、质子-磷以及磷-碳耦合常数。这些信息可在合理的测量时间内获得，并为DNA结构的详细描述提供了有价值的信息。
相似文献
1
Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.天然丰度下含异核的DNA的异核核磁共振：耦合常数的便捷归属与提取
Nucleic Acids Res. 1992 Sep 25;20(18):4747-51. doi: 10.1093/nar/20.18.4747.
2
Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.采用氢检测二维异核相关光谱法对短DNA双链体的碳-13核磁共振谱进行归属。
Nucleic Acids Res. 1987 Jan 12;15(1):267-75. doi: 10.1093/nar/15.1.267.
3
13C-NMR of the deoxyribose sugars in four DNA oligonucleotide duplexes: assignment and structural features.
Biochemistry. 1994 Mar 8;33(9):2430-40. doi: 10.1021/bi00175a011.
4
Two- and three-dimensional 31P-driven NMR procedures for complete assignment of backbone resonances in oligodeoxyribonucleotides.用于寡脱氧核糖核苷酸主链共振完全归属的二维和三维磷驱动核磁共振方法。
J Biomol NMR. 1993 Sep;3(5):577-95. doi: 10.1007/BF00174611.
5
Preparation and heteronuclear 2D NMR spectroscopy of a DNA dodecamer containing a thymidine residue with a uniformly 13C-labeled deoxyribose ring.含一个具有均匀13C标记脱氧核糖环的胸苷残基的DNA十二聚体的制备及异核二维核磁共振波谱分析
J Biomol NMR. 1994 Jul;4(4):581-6. doi: 10.1007/BF00156622.
6
Heteronuclear two-dimensional 15N- and 13C-NMR studies of DNA oligomers and their netropsin complexes using indirect proton detection.使用间接质子检测对DNA寡聚物及其纺锤菌素复合物进行异核二维15N和13C核磁共振研究。
Biopolymers. 1991 Jan;31(1):45-55. doi: 10.1002/bip.360310105.
7
Hairpin loops consisting of single adenine residues closed by sheared A.A and G.G pairs formed by the DNA triplets AAA and GAG: solution structure of the d(GTACAAAGTAC) hairpin.由DNA三联体AAA和GAG形成的由剪切的A·A和G·G对封闭的单腺嘌呤残基组成的发夹环：d(GTACAAAGTAC)发夹的溶液结构
J Mol Biol. 1996 Dec 20;264(5):981-1001. doi: 10.1006/jmbi.1996.0691.
8
Two-dimensional 1H and 31P NMR spectra and restrained molecular dynamics structure of an oligodeoxyribonucleotide duplex refined via a hybrid relaxation matrix procedure.通过混合弛豫矩阵方法优化的寡脱氧核糖核苷酸双链体的二维¹H和³¹P NMR谱及受限分子动力学结构。
J Biomol Struct Dyn. 1990 Oct;8(2):253-94. doi: 10.1080/07391102.1990.10507805.
9
Filtering methods for selection of singlet and doublet signals in NMR spectra of DNA oligomers.用于选择DNA寡聚物核磁共振谱中单重态和双重态信号的过滤方法。
J Biomol NMR. 1994 Mar;4(2):181-91. doi: 10.1007/BF00175246.
10
13C NMR of the bases of three DNA oligonucleotide duplexes: assignment methods and structural features.三种DNA寡核苷酸双链体碱基的13C核磁共振：归属方法与结构特征
Biochemistry. 1988 Oct 4;27(20):7902-9. doi: 10.1021/bi00420a048.
引用本文的文献
1
Stereospecific assignment of H5' and H5″ in a (5'R)-/(5'S)-deuterium- labeled DNA decamer for(3) J (HH) determination and unambiguous NOE assignments.用于（3）J（HH）测定和明确 NOE 分配的（5'R）/（5'S）-氘标记 DNA 十聚体中 H5'和 H5″的立体专一性分配。
J Biomol NMR. 1998 Jan;11(1):103-9. doi: 10.1023/A:1008286925516.
2
Tuning of conformational preorganization in model 2',5'- and 3',5'-linked oligonucleotides by 3'- and 2'-O-methoxyethyl modification.通过3'-和2'-O-甲氧基乙基修饰对2',5'-和3',5'-连接的寡核苷酸中构象预组织的调控
Nucleic Acids Res. 2003 Apr 15;31(8):2066-76. doi: 10.1093/nar/gkg305.
3
Determination of the glycosidic torsion angles in uniformly 13C-labeled nucleic acids from vicinal coupling constants 3J(C2)/4-H1' and 3J(C6)/8-H1'.通过邻位偶合常数3J(C2)/4-H1'和3J(C6)/8-H1'测定均匀13C标记核酸中的糖苷扭转角。
J Biomol NMR. 2002 May;23(1):1-12. doi: 10.1023/a:1015389118506.
4
Enhanced loop DNA folding induced by thymine-CH3 group contact and perpendicular guanine-thymine interaction.胸腺嘧啶-CH3基团接触和鸟嘌呤-胸腺嘧啶垂直相互作用诱导的增强型环状DNA折叠
J Biomol NMR. 2001 Jan;19(1):33-48. doi: 10.1023/a:1008372701261.
5
Natural abundance heteronuclear NMR studies of the T3 mini-loop hairpin in the terminal repeat of the adenoassociated virus 2.腺相关病毒2末端重复序列中T3小环发夹结构的天然丰度异核核磁共振研究。
J Biomol NMR. 2000 May;17(1):1-16. doi: 10.1023/a:1008380031690.
6
Long-range imino proton-13C J-couplings and the through-bond correlation of imino and non-exchangeable protons in unlabeled DNA.未标记DNA中长程亚氨基质子-13C J耦合以及亚氨基与非交换质子的键连相关性
J Biomol NMR. 2000 Feb;16(2):175-8. doi: 10.1023/a:1008355231085.
7
Conformation of the circular dumbbell d<pCGC-TT-GCG-TT>: structure determination and molecular dynamics.环状哑铃状d<pCGC-TT-GCG-TT>的构象：结构测定与分子动力学
J Biomol NMR. 1995 Dec;6(4):403-22. doi: 10.1007/BF00197639.
8
Preparation and heteronuclear 2D NMR spectroscopy of a DNA dodecamer containing a thymidine residue with a uniformly 13C-labeled deoxyribose ring.含一个具有均匀13C标记脱氧核糖环的胸苷残基的DNA十二聚体的制备及异核二维核磁共振波谱分析
J Biomol NMR. 1994 Jul;4(4):581-6. doi: 10.1007/BF00156622.
9
Structural characterization of d(CAACCCGTTG) and d(CAACGGGTTG) mini-hairpin loops by heteronuclear NMR: the effects of purines versus pyrimidines in DNA hairpins.通过异核核磁共振对d(CAACCCGTTG)和d(CAACGGGTTG)微型发夹环进行结构表征：DNA发夹中嘌呤与嘧啶的影响
Nucleic Acids Res. 1995 Apr 11;23(7):1260-8. doi: 10.1093/nar/23.7.1260.
10
Novel three-dimensional 1H-13C-31P triple resonance experiments for sequential backbone correlations in nucleic acids.
J Biomol NMR. 1995 Apr;5(3):315-20. doi: 10.1007/BF00211759.
本文引用的文献
1
Carbon-13 NMR in conformational analysis of nucleic acid fragments. Heteronuclear chemical shift correlation spectroscopy of RNA constituents.核酸片段构象分析中的碳-13核磁共振。RNA成分的异核化学位移相关光谱学。
Nucleic Acids Res. 1983 Oct 25;11(20):7215-30. doi: 10.1093/nar/11.20.7215.
2
Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.采用氢检测二维异核相关光谱法对短DNA双链体的碳-13核磁共振谱进行归属。
Nucleic Acids Res. 1987 Jan 12;15(1):267-75. doi: 10.1093/nar/15.1.267.
3
13C NMR assignments of the protonated carbons of [d(TAGCGCTA)]2 by two-dimensional proton-detected heteronuclear correlation.通过二维质子检测异核相关对[d(TAGCGCTA)]₂质子化碳的¹³C核磁共振归属
J Biomol Struct Dyn. 1988 Apr;5(5):1089-99. doi: 10.1080/07391102.1988.10506450.
4
13C NMR of the bases of three DNA oligonucleotide duplexes: assignment methods and structural features.三种DNA寡核苷酸双链体碱基的13C核磁共振：归属方法与结构特征
Biochemistry. 1988 Oct 4;27(20):7902-9. doi: 10.1021/bi00420a048.
5
Heteronuclear nuclear magnetic resonance experiments for studies of protein conformation.
Methods Enzymol. 1989;176:93-113. doi: 10.1016/0076-6879(89)76007-9.
6
Detection of insensitive nuclei.
Methods Enzymol. 1989;176:134-50. doi: 10.1016/0076-6879(89)76009-2.
7
Determination of the DNA sugar pucker using 13C NMR spectroscopy.使用碳-13核磁共振光谱法测定DNA糖的构象。
Biochemistry. 1989 Nov 28;28(24):9372-8. doi: 10.1021/bi00450a018.
8
Concerted two-dimensional NMR approaches to hydrogen-1, carbon-13, and nitrogen-15 resonance assignments in proteins.
Biochemistry. 1989 Jan 10;28(1):230-6. doi: 10.1021/bi00427a032.
9
A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.一种用于蛋白质1H、13C和15N谱顺序归属的新方法：异核三共振三维核磁共振光谱法。应用于钙调蛋白。
Biochemistry. 1990 May 15;29(19):4659-67. doi: 10.1021/bi00471a022.
10
Heteronuclear three-dimensional NMR spectroscopy of isotopically labelled biological macromolecules.同位素标记生物大分子的异核三维核磁共振光谱学。
Q Rev Biophys. 1990 May;23(2):97-131. doi: 10.1017/s0033583500005515.
Zotero 插件