Kojima C, Kyogoku Y
Institute for Protein Research, Osaka University, Japan.
J Biomol NMR. 1994 Mar;4(2):181-91. doi: 10.1007/BF00175246.
The base proton (purine H8 and pyrimidine H6) resonances are key signals for the assignment of the proton resonances of DNA oligomers. They are classified into two groups, i.e., cytosine H6 signals, observed as doublets, and the other base proton signals, observed as singlets. Here we propose some experiments for distinguishing the cytosine H6 signals from the other base proton signals. Moreover, the ability of signal selection and the sensitivity as to signal detection were compared for all experiments, and the optimum conditions for spectral measurements were surveyed. Some of the experiments were employed as the NOESY detection pulse. Previously proposed experiments, such as HOENOE and HAL, were also used in the comparison.
碱基质子（嘌呤H8和嘧啶H6）共振是确定DNA寡聚物质子共振的关键信号。它们被分为两组，即观察为双峰的胞嘧啶H6信号和观察为单峰的其他碱基质子信号。在此，我们提出了一些用于区分胞嘧啶H6信号与其他碱基质子信号的实验。此外，对所有实验的信号选择能力和信号检测灵敏度进行了比较，并研究了光谱测量的最佳条件。其中一些实验被用作NOESY检测脉冲。之前提出的实验，如HOENOE和HAL，也用于比较。
