采用氢检测二维异核相关光谱法对短DNA双链体的碳-13核磁共振谱进行归属。
Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.
作者信息
Leupin W, Wagner G, Denny W A, Wüthrich K
出版信息
Nucleic Acids Res. 1987 Jan 12;15(1):267-75. doi: 10.1093/nar/15.1.267.
Abstract
Proton-detected 1H-13C heteronuclear correlated spectroscopy [( 1H,13C]-COSY) was used to establish relations between the carbon-13 and proton nuclear magnetic resonance chemical shifts in the hexadeoxynucleoside pentaphosphate d-(GCATGC)2. Using the previously established sequence-specific proton NMR assignments, sequence-specific assignments were thus obtained for nearly all proton-bearing carbons. This approach offers a new criterion for distinguishing between the proton NMR lines of purines and pyrimidines, based on the different proton-carbon-13 coupling constants. Furthermore, the adenine ring carbon 2 has a unique carbon-13 chemical shift, which enables a straightforward identification of the adenine C2H resonances by [1H,13C]-COSY.
摘要
质子检测的1H-13C异核相关光谱法[（1H，13C）-COSY]用于建立十六脱氧核苷五磷酸d-（GCATGC）2中碳-13和质子核磁共振化学位移之间的关系。利用先前建立的序列特异性质子核磁共振归属，从而获得了几乎所有含质子碳的序列特异性归属。基于不同的质子-碳-13耦合常数，该方法为区分嘌呤和嘧啶的质子核磁共振谱线提供了一个新的标准。此外，腺嘌呤环碳2具有独特的碳-13化学位移，这使得通过[1H，13C]-COSY能够直接鉴定腺嘌呤C2H共振。
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