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本文引用的文献

1
13C NMR study of conformation and mobility of 145-base-pair poly(dA-dT) . poly(dA-dT) in solution.溶液中145个碱基对的聚（dA-dT）·聚（dA-dT）的构象和流动性的13C核磁共振研究。
Eur J Biochem. 1981 Nov;120(2):309-12. doi: 10.1111/j.1432-1033.1981.tb05705.x.
2
Two-dimensional proton nuclear magnetic resonance investigation of the synthetic deoxyribonucleic acid decamer d(ATATCGATAT)2.合成脱氧核糖核酸十聚体d(ATATCGATAT)₂的二维质子核磁共振研究
Biochemistry. 1983 Dec 6;22(25):5943-51. doi: 10.1021/bi00294a038.
3
Conformation and dynamics of short DNA duplexes: (dC-dG)3 and (dC-dG)4.短DNA双链体的构象与动力学：(dC-dG)3和(dC-dG)4
J Biomol Struct Dyn. 1984 Jun;1(6):1373-86. doi: 10.1080/07391102.1984.10507526.
4
Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methods.使用二维核磁共振方法对d（C-G-C-G-A-A-T-T-C-G-C-G）的非交换质子共振进行归属
J Mol Biol. 1983 Dec 15;171(3):319-36. doi: 10.1016/0022-2836(83)90096-7.
5
Two-dimensional 1H NMR study of the lambda operator site OL1: a sequential assignment strategy and its application.λ 操纵基因位点 OL1 的二维¹H NMR 研究：一种序列归属策略及其应用
Proc Natl Acad Sci U S A. 1984 Jan;81(1):130-4. doi: 10.1073/pnas.81.1.130.
6
Carbon-13 NMR in conformational analysis of nucleic acid fragments. Heteronuclear chemical shift correlation spectroscopy of RNA constituents.核酸片段构象分析中的碳-13核磁共振。RNA成分的异核化学位移相关光谱学。
Nucleic Acids Res. 1983 Oct 25;11(20):7215-30. doi: 10.1093/nar/11.20.7215.
7
Sequence-specific assignment of the backbone 1H-and 31P-NMR lines in a short DNA duplex with homo- and heteronuclear correlated spectroscopy.利用同核和异核相关光谱对短DNA双链体中骨架1H和31P-NMR谱线进行序列特异性归属。
Biopolymers. 1985 Dec;24(12):2371-80. doi: 10.1002/bip.360241214.
8
Carbon-13 NMR in conformational analysis of nucleic acid fragments. 4. The torsion angle distribution about the C3'-O3' bond in DNA constituents.碳-13核磁共振在核酸片段构象分析中的应用。4. DNA组分中围绕C3'-O3'键的扭转角分布。
Nucleic Acids Res. 1985 Feb 11;13(3):927-42. doi: 10.1093/nar/13.3.927.
9
NMR studies of chromomycin A3 interaction with DNA.放线菌素A3与DNA相互作用的核磁共振研究。
Biochemistry. 1985 Nov 19;24(24):6887-93. doi: 10.1021/bi00345a022.

采用氢检测二维异核相关光谱法对短DNA双链体的碳-13核磁共振谱进行归属。

Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.

作者信息

Leupin W, Wagner G, Denny W A, Wüthrich K

出版信息

Nucleic Acids Res. 1987 Jan 12;15(1):267-75. doi: 10.1093/nar/15.1.267.

DOI:10.1093/nar/15.1.267
PMID:3822804
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340409/
Abstract

Proton-detected 1H-13C heteronuclear correlated spectroscopy [( 1H,13C]-COSY) was used to establish relations between the carbon-13 and proton nuclear magnetic resonance chemical shifts in the hexadeoxynucleoside pentaphosphate d-(GCATGC)2. Using the previously established sequence-specific proton NMR assignments, sequence-specific assignments were thus obtained for nearly all proton-bearing carbons. This approach offers a new criterion for distinguishing between the proton NMR lines of purines and pyrimidines, based on the different proton-carbon-13 coupling constants. Furthermore, the adenine ring carbon 2 has a unique carbon-13 chemical shift, which enables a straightforward identification of the adenine C2H resonances by [1H,13C]-COSY.

摘要

质子检测的1H-13C异核相关光谱法[（1H，13C）-COSY]用于建立十六脱氧核苷五磷酸d-（GCATGC）2中碳-13和质子核磁共振化学位移之间的关系。利用先前建立的序列特异性质子核磁共振归属，从而获得了几乎所有含质子碳的序列特异性归属。基于不同的质子-碳-13耦合常数，该方法为区分嘌呤和嘧啶的质子核磁共振谱线提供了一个新的标准。此外，腺嘌呤环碳2具有独特的碳-13化学位移，这使得通过[1H，13C]-COSY能够直接鉴定腺嘌呤C2H共振。