Benítez-Bribiesca L, Guevara R, Ruiz M T, Martínez G, Rodríguez-Cuevas S
Unidad de Investigación Clínica en Enfermedades Oncológicas, Hospital de Oncología, México.
Pathol Res Pract. 1992 Jun;188(4-5):461-5. doi: 10.1016/S0344-0338(11)80038-5.
Different histoscores combining the number of positive cells and the intensity of staining have been used to evaluate the estrogen receptor immunocytochemical assay (ER-ICA). Our aim was to investigate if the simple estimation of the amount of positive cells could be sufficient for the semiquantitative analysis of ER-ICA. Tissue from 51 women with ductal breast carcinoma was used. Half of each sample was processed with the quantitative assay (ER-EIA) and the other half with ER-ICA. Microscopical analysis was performed by two independent observers and classified on a simple scale from 0 to 4+. With EIA 31 cases (60.78%) were positive and 20 (39.21%) negative. With ER-ICA 29 (56.86%) had immunostaining, whereas 22 (43.13%) did not. 95.83% of the ER-ICA positive cases and 77.7% of ER-ICA negative had a good correlation with EIA values. Statistical analysis showed a high degree of correlation (r = 0.88 p 0.001). Hence, simple semiquantitative estimation in ER-ICA is sufficient to provide useful information for clinical use about ER content in tissue sections.
结合阳性细胞数量和染色强度的不同组织学评分已被用于评估雌激素受体免疫细胞化学检测（ER-ICA）。我们的目的是研究阳性细胞数量的简单估计对于ER-ICA的半定量分析是否足够。使用了51例乳腺导管癌女性的组织。每个样本的一半用定量检测法（ER-EIA）处理，另一半用ER-ICA处理。由两名独立观察者进行显微镜分析，并按从0到4+的简单量表进行分类。采用EIA法，31例（60.78%）为阳性，20例（39.21%）为阴性。采用ER-ICA法，29例（56.86%）有免疫染色，22例（43.13%）无免疫染色。95.83%的ER-ICA阳性病例和77.7%的ER-ICA阴性病例与EIA值具有良好的相关性。统计分析显示高度相关（r = 0.88，p < 0.001）。因此，ER-ICA中的简单半定量估计足以提供关于组织切片中ER含量的临床有用信息。
