Dal Cin P, Aly M S, Delabie J, Ceuppens J L, Van Gool S, Van Damme B, Baert L, Van Poppel H, Van den Berghe H
Center for Human Genetics, University of Leuven, Belgium.
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9744-8. doi: 10.1073/pnas.89.20.9744.
We performed conventional cytogenetic analysis and fluorescence in situ hybridization in short-term cultures of normal and neoplastic kidney tissues. Cell populations carrying an extra chromosome 7 or an extra chromosome 10 as the only chromosome change could be identified in kidney tumors, mostly renal cell carcinomas, and in the surrounding kidney tissue, but not in nonneoplastic kidneys. To identify the type of cells displaying these aneuploidies, we performed in situ hybridization (ISH) with probes specific for the centromeric region of chromosomes 7 and 10 on frozen kidney tissue sections. Trisomy 7 and trisomy 10 were restricted to infiltrating inflammatory cells in the tumor as well as in the surrounding tissue. Trisomy 7 and trisomy 10 were also found in subpopulations of peripheral blood T cells of cancer patients and of normal individuals, as well as in the thymus of five normal fetuses (21-29 weeks), but not in noninvaded reactive lymph node sections of patients without malignancy. When lymphocytes were enriched from kidney tumors and surrounding tissue by either Ficoll/Hypaque density gradient or immunomagnetic selection with anti-CD3, anti-CD4, or anti-CD8 monoclonal antibodies, it was confirmed that they contained a high percentage of trisomy 7 and trisomy 10 cells. Further proof for T-lymphocyte origin of the trisomy 7 and trisomy 10 cells was obtained by simultaneous staining of lymphocytes isolated from tumor tissue with anti-CD3, anti-CD4, and anti-CD8 monoclonal antibodies and ISH. We conclude that trisomy 7 and trisomy 10, found in renal carcinomas and surrounding kidney tissue, characterize subpopulations of tumor-infiltrating lymphocytes. The biologic significance of this phenomenon is unknown and requires further investigation.
我们对正常和肿瘤性肾组织的短期培养物进行了常规细胞遗传学分析和荧光原位杂交。在肾肿瘤（主要是肾细胞癌）及其周围肾组织中，可以鉴定出携带额外的7号染色体或额外的10号染色体作为唯一染色体变化的细胞群体，但在非肿瘤性肾脏中未发现。为了确定显示这些非整倍体的细胞类型，我们在冷冻的肾组织切片上用针对7号和10号染色体着丝粒区域的探针进行了原位杂交（ISH）。7号染色体三体和10号染色体三体仅限于肿瘤及其周围组织中的浸润性炎症细胞。在癌症患者和正常个体的外周血T细胞亚群中，以及在5例正常胎儿（21 - 29周）的胸腺中也发现了7号染色体三体和10号染色体三体，但在无恶性肿瘤患者的未受侵袭的反应性淋巴结切片中未发现。当通过Ficoll/Hypaque密度梯度或用抗CD3、抗CD4或抗CD8单克隆抗体进行免疫磁选从肾肿瘤和周围组织中富集淋巴细胞时，证实它们含有高比例的7号染色体三体和10号染色体三体细胞。通过用抗CD3、抗CD4和抗CD8单克隆抗体以及ISH对从肿瘤组织中分离的淋巴细胞进行同时染色，获得了7号染色体三体和10号染色体三体细胞起源于T淋巴细胞的进一步证据。我们得出结论，在肾癌及其周围肾组织中发现的7号染色体三体和10号染色体三体是肿瘤浸润淋巴细胞亚群的特征。这种现象的生物学意义尚不清楚，需要进一步研究。
