Matsumoto T, Cyong J C, Yamada H
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Planta Med. 1992 Jun;58(3):255-8. doi: 10.1055/s-2006-961447.
Immune complex binding to macrophages was enhanced by treatment with an E. kansui extract. Systematic fractionation of the extract led to the characterization of 3-O-(2'E,4'Z-decadienoyl)- and 3-O-(2,3-dimethylbutyryl)-13- O-n-dodecanoyl-13-hydroxyingenol as the active principles. Immune complex binding to macrophages by the action of these compounds increased in a dose-dependent manner. When each ingenol (10 nM) was added to the separated culture medium, the immune complex binding ability of macrophages increased up to 2-fold, respectively. Scatchard analysis showed the enhanced expression of the Fc-receptor for gamma-globulin by the action of each ingenol to macrophages. This Fc-receptor upregulation was dependent on RNA synthesis, suggesting a possible de novo synthesis.
用甘遂提取物处理可增强免疫复合物与巨噬细胞的结合。对提取物进行系统分级分离，鉴定出3 - O -（2'E，4'Z - 癸二烯酰基）-和3 - O -（2,3 - 二甲基丁酰基）- 13 - O - 正十二烷酰基 - 13 - 羟基大戟醇为活性成分。这些化合物作用下免疫复合物与巨噬细胞的结合呈剂量依赖性增加。当将每种大戟醇（10 nM）添加到分离的培养基中时，巨噬细胞的免疫复合物结合能力分别提高了2倍。Scatchard分析表明，每种大戟醇作用于巨噬细胞后，γ-球蛋白的Fc受体表达增强。这种Fc受体上调依赖于RNA合成，提示可能存在从头合成。
