MCDOUGAL D B, SCHIMKE R T, JONES E M, TOUHILL E
J Gen Physiol. 1964 Jan;47(3):419-32. doi: 10.1085/jgp.47.3.419.
Methods for measurement of glyceraldehyde-P dehydrogenase, triose-P isomerase, fructose 1,6-diphosphate aldolase, and the DPN-linked and flavin-linked alpha-glycero-P dehydrogenases in small amounts of tissue have been worked out. These enzymes have been measured in ten tracts in rabbit central nervous system. The activities of all the enzymes measured, except the flavin-linked alpha-glycero-P dehydrogenase, are present in larger amounts in lightly myelinated than in heavily myelinated tracts, but are relatively low in fibrillar layer of olfactory bulb, which is unmyelinated. Aldolase, like P-fructokinase (measured previously), is especially low in fibrillar layer. Taken together with relatively high 6-P-gluconate dehydrogenase activity found earlier this supports the hypothesis that the pentose-P shunt is particularly active in this tract. The activity of DPN-linked alpha-glycero-P dehydrogenase is inversely proportional to the lipid content of the myelinated tracts, which suggests that its primary role is not related to lipid synthesis in adult brain. The activities of flavin-linked alpha-glycero-P dehydrogenase are unrelated to those of the DPN-linked enzyme, which is contrary to expectation if the two enzymes function as partners in the "alpha-glycerophosphate shuttle."
已研究出在少量组织中测量甘油醛 - 3 - 磷酸脱氢酶、磷酸丙糖异构酶、果糖1,6 - 二磷酸醛缩酶以及与二磷酸吡啶核苷酸（DPN）相连和与黄素相连的α - 甘油磷酸脱氢酶的方法。已在兔中枢神经系统的十条神经束中对这些酶进行了测量。除了与黄素相连的α - 甘油磷酸脱氢酶外，所测量的所有酶的活性在轻度髓鞘化的神经束中比在重度髓鞘化的神经束中含量更高，但在无髓鞘的嗅球纤维层中相对较低。醛缩酶，就像之前测量的磷酸果糖激酶一样，在纤维层中特别低。结合早前发现的相对较高的6 - 磷酸葡萄糖酸脱氢酶活性，这支持了戊糖磷酸途径在该神经束中特别活跃的假说。与DPN相连的α - 甘油磷酸脱氢酶的活性与髓鞘化神经束的脂质含量成反比，这表明其主要作用在成体大脑中与脂质合成无关。与黄素相连的α - 甘油磷酸脱氢酶的活性与与DPN相连的酶的活性无关，如果这两种酶在“α - 甘油磷酸穿梭”中作为伙伴发挥作用，这与预期相反。
