Dixon H B, Sparkes M J
Biochem J. 1974 Sep;141(3):715-9. doi: 10.1042/bj1410715.
Analogues of dihydroxyacetone phosphate and of 3-phosphoglycerate were made in which the phosphate group, -O-PO(3)H(2), is replaced by the phosphonomethyl group, -CH(2)-PO(3)H(2). The analogue of dihydroxyacetone phosphate is a substrate for aldolase and glycerol 1-phosphate dehydrogenase (Stribling, 1974), but not for triose phosphate isomerase. The analogue of 3-phosphoglycerate oxidizes NADH under the combined action of 3-phosphoglycerate kinase and glyceraldehyde 3-phosphate dehydrogenase if ATP is added. Thus four out of the five glycolytic enzymes tested handle the phosphonomethyl compounds like the natural phosphates.
制备了磷酸二羟丙酮和3-磷酸甘油酸的类似物，其中磷酸基团-O-PO(3)H(2)被膦酰甲基-CH(2)-PO(3)H(2)取代。磷酸二羟丙酮类似物是醛缩酶和甘油1-磷酸脱氢酶的底物（斯特林，1974年），但不是磷酸丙糖异构酶的底物。如果添加ATP，3-磷酸甘油酸类似物在3-磷酸甘油酸激酶和甘油醛3-磷酸脱氢酶的联合作用下氧化NADH。因此，所测试的五种糖酵解酶中有四种处理膦酰甲基化合物的方式与天然磷酸盐类似。
