D-甘油醛3-磷酸在与D-甘油醛3-磷酸脱氢酶、醛缩酶和磷酸丙糖异构酶反应中的活性化学状态。

Trentham D R, McMurray C H, Pogson C I

Biochem J. 1969 Aug;114(1):19-24. doi: 10.1042/bj1140019.

Glyceraldehyde 3-phosphate exists as the geminal diol and the free aldehyde in the molar ratio 29:1 in aqueous solution. The rate constant of the conversion of diol into aldehyde is 8.7x10(-2)sec.(-1) in the pH range 7.3-8.6 at 20 degrees . The free aldehyde is the substrate for d-glyceraldehyde 3-phosphate dehydrogenase. Over a wide concentration range of enzyme the rate of conversion of diol into aldehyde is the rate-limiting process in the catalytic oxidation of d-glyceraldehyde 3-phosphate by NAD(+). Aldolase and triose phosphate isomerase both liberate d-glyceraldehyde 3-phosphate as the aldehyde. This suggests that the relatively slow diol-aldehyde interconversion does not restrict the rate of glycolysis.

在水溶液中，3-磷酸甘油醛以偕二醇和游离醛的形式存在，摩尔比为29:1。在20摄氏度、pH值7.3 - 8.6的范围内，二醇转化为醛的速率常数为8.7×10⁻²秒⁻¹。游离醛是d-3-磷酸甘油醛脱氢酶的底物。在较宽的酶浓度范围内，二醇转化为醛的速率是NAD⁺催化氧化d-3-磷酸甘油醛过程中的限速步骤。醛缩酶和磷酸丙糖异构酶都释放出醛形式的d-3-磷酸甘油醛。这表明相对缓慢的二醇-醛相互转化不会限制糖酵解的速率。