Laszlo A, Baer H H, Goren M B, Handzel V, Barrera L, de Kantor I N
Laboratory Centre for Disease Control, Health & Welfare Canada, Ottawa.
Res Microbiol. 1992 Feb;143(2):217-23. doi: 10.1016/0923-2508(92)90011-c.
A number of glycolipids were evaluated in an ELISA test for their serodiagnostic usefulness in tuberculosis. One hundred and twelve (112) sera belonging to bacteriologically confirmed TB patients, patients with pathologies other than tuberculosis and healthy individuals were examined against several synthetic "mirror" pseudo cord factors (analogues of trehalose-6,6'-dimycolate or TDM) using natural cord factor and another recently described natural glycolipid (SL-IV) of Mycobacterium tuberculosis as control antigens. Analysis of the results shows that all synthetic "mirror" pseudo cord factors, except one with a short 8-carbon chain, were better recognized by the sera of tuberculosis patients than natural cord factor, with sensitivity and specificity values in the ELISA test similar to those reported for M. tuberculosis species-specific SL-IV. Of all antigens tested in this study, BDA. TDA, a bis(N,N-dioctadecylamide) of "trehalose dicarboxylic acid", [(alpha-D-glucopyranosyluronic acid) (alpha-D-glucopyranosiduranic acid)], showed the highest serodiagnostic discriminating power (93% sensitivity and specificity). We postulate that either these artificial molecules are cross-reactants of similarly structured native glycolipids of M. tuberculosis or that they bear closer resemblance to actual phagosome-lysosome-modified antigens than to native mycobacterial ones.
在一项酶联免疫吸附测定（ELISA）试验中，对多种糖脂在结核病血清诊断中的效用进行了评估。使用天然索状因子和另一种最近描述的结核分枝杆菌天然糖脂（SL-IV）作为对照抗原，针对几种合成的“镜像”假索状因子（海藻糖-6,6'-二霉菌酸酯或TDM的类似物），对112份血清进行了检测，这些血清分别来自经细菌学确诊的结核病患者、患有结核病以外其他疾病的患者以及健康个体。结果分析表明，除一种具有短8碳链的合成“镜像”假索状因子外，所有此类因子在ELISA试验中被结核病患者血清识别的效果均优于天然索状因子，其敏感性和特异性值与报道的结核分枝杆菌种特异性SL-IV相似。在本研究中测试的所有抗原中，“海藻糖二羧酸”的双（N,N-二十八烷基酰胺）[（α-D-吡喃葡萄糖醛酸）（α-D-吡喃葡萄糖醛酸）]，即BDA.TDA，显示出最高的血清诊断鉴别能力（敏感性和特异性均为93%）。我们推测，要么这些人工分子是结核分枝杆菌结构相似的天然糖脂的交叉反应物，要么它们与实际的吞噬体-溶酶体修饰抗原的相似性比与天然分枝杆菌抗原的相似性更高。
