用于检测结核病患者抗二酰海藻糖抗体的斑点印迹法。
Dot blot assay for detection of antidiacyltrehalose antibodies in tuberculous patients.
作者信息
Vera-Cabrera L, Rendon A, Diaz-Rodriguez M, Handzel V, Laszlo A
机构信息
Servicios de Dermatología, Hospital Universitario "Dr. José Eleuterio González," Monterrey, Nuevo León, México.
出版信息
Clin Diagn Lab Immunol. 1999 Sep;6(5):686-9. doi: 10.1128/CDLI.6.5.686-689.1999.
Abstract
A simple dot blot test with diacyltrehalose (DAT) as the antigen was developed to detect anti-DAT antibodies in tuberculous patients. To enhance antigen-antibody reaction detection, rabbit serum raised against human immunoglobulins was used prior to incubation with a protein A-colloidal gold complex. With the dot blot system, it was possible to obtain a sensitivity similar to that of enzyme-linked immunosorbent assay (ELISA) and a specificity of 97.14%, versus a specificity of 94.29% by the ELISA. We conclude that this simple and fast assay could be used in places where ELISA equipment is not easy available and that it might also be applicable with other Mycobacterium tuberculosis immunogenic antigens.
摘要
开发了一种以二酰海藻糖（DAT）为抗原的简单斑点印迹试验，用于检测结核病患者中的抗DAT抗体。为了增强抗原-抗体反应检测，在与蛋白A-胶体金复合物孵育之前，使用了针对人免疫球蛋白产生的兔血清。使用斑点印迹系统，可以获得与酶联免疫吸附测定（ELISA）相似的灵敏度，特异性为97.14%，而ELISA的特异性为94.29%。我们得出结论，这种简单快速的检测方法可用于不易获得ELISA设备的地方，并且也可能适用于其他结核分枝杆菌免疫原性抗原。
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