FUDENBERG H H, DREWS G, NISONOFF A
J Exp Med. 1964 Jan 1;119(1):151-66. doi: 10.1084/jem.119.1.151.
Hybrid, bivalent antibody molecules bearing specific combining sites for both ovalbumin and bovine gamma globulin were produced by reoxidation of a mixture of the 3.5S fragments of the two specifically purified antibodies. The dual specificity and the properties of the hybrid antibody were demonstrated by mixed agglutination and two stage agglutination experiments, and by test systems utilizing inhibition of agglutination or dispersal of agglutinates followed by antiglobulin reactions.
通过对两种特异性纯化抗体的3.5S片段混合物进行再氧化,产生了对卵清蛋白和牛γ球蛋白均具有特异性结合位点的杂合二价抗体分子。通过混合凝集和两阶段凝集实验,以及利用凝集抑制或凝集物分散后进行抗球蛋白反应的测试系统,证明了杂合抗体的双重特异性和特性。