Da Silva L B, Trebes J E, Balhorn R, Mrowka S, Anderson E, Attwood D T, Barbee T W, Brase J, Corzett M, Gray J
Department of Physics, University of California, Berkeley 94720.
Science. 1992 Oct 9;258(5080):269-71. doi: 10.1126/science.1411525.
The development of high brightness and short pulse width (< 200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show approximately 500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.
高亮度且脉宽较短（<200皮秒）的X射线激光器的发展，如今为生物学家提供了在水环境中对标本进行高分辨率成像的可能性，而不会出现与自然运动和化学侵蚀相关的模糊效应。作为发展此类X射线显微镜功能的一步，一台波长为44.83埃的钽X射线激光器与一个X射线波带片透镜一起，用于对未标记和选择性金标记的干燥大鼠精子细胞核进行成像。观察到的图像显示出约500埃的特征，阐明了X射线显微镜在确定化学成分方面的重要性，并提供了有关精子染色质组织均匀性和精子染色质水合程度的信息。
