Tomie T, Shimizu H, Majima T, Yamada M, Kanayama T, Kondo H, Yano M, Ono M
Electrotechnical Laboratory, Ibaraki, Japan.
Science. 1991 May 3;252(5006):691-3. doi: 10.1126/science.2024121.
The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subnanosecond [corrected] x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height.
通过使用强闪光X射线源和先进的X射线图像读取技术，利用X射线显微镜对水中活体标本进行成像的效果可得到极大提升。来自激光产生等离子体的亚纳秒[已修正]X射线脉冲被用于记录活体海胆精子在X射线抗蚀剂中的X射线图像。通过原子力显微镜以高分辨率观察到抗蚀剂浮雕。精子头部的内部结构清晰可见，并且根据浮雕高度估算了鞭毛中的碳密度。
