在苄索氯铵存在下百日咳疫苗的稳定性
STABILIZATION OF PERTUSSIS VACCINE IN THE PRESENCE OF BENZETHONIUM CHLORIDE.
作者信息
OLSON B H, ELDERING G, GRAHAM B
出版信息
J Bacteriol. 1964 Mar;87(3):543-6. doi: 10.1128/jb.87.3.543-546.1964.
Olson, B. H. (Division of Laboratories, Michigan Department of Health, Lansing), Grace Eldering, and Bernice Graham. Stabilization of pertussis vaccine in the presence of benzethonium chloride. J. Bacteriol. 87:543-546. 1964.-Data are presented showing that pertussis vaccine preserved with benzethonium chloride (BC; Phemerol) was inactivated during storage. BC-preserved vaccine stored at 37 C showed no measurable mouse-protective potency at 16 weeks. That stored at 0 to 4 C lost approximately 80% of its potency within 1 year. Treatment of pertussis vaccines with aluminum, calcium, magnesium, choline, or dl-lysine before the addition of the BC prevented its uptake by the cells. Pertussis vaccines pretreated with 0.004 m Ca(++) or 0.0004 m Al(+++) retained 70% of the initial potency after 42 weeks of storage at 37 C. Similar vaccines showed no loss of protective antigens when stored for 1 year at 0 to 4 C.
奥尔森，B. H.（密歇根州卫生部实验室分部，兰辛）、格蕾丝·埃尔德林和伯尼斯·格雷厄姆。在苄索氯铵存在下百日咳疫苗的稳定性。《细菌学杂志》87:543 - 546。1964年。——所呈现的数据表明，用苄索氯铵（BC；酚汞）保存的百日咳疫苗在储存期间会失活。在37℃储存的用BC保存的疫苗在16周时未显示出可测量的小鼠保护效力。在0至4℃储存的疫苗在1年内失去了约80%的效力。在添加BC之前用铝、钙、镁、胆碱或dl - 赖氨酸处理百日咳疫苗可防止细胞摄取它。用0.004 m Ca(++)或0.0004 m Al(+++)预处理的百日咳疫苗在37℃储存42周后保留了70%的初始效力。类似的疫苗在0至4℃储存1年时未显示出保护性抗原的损失。
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