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胸腺嘧啶和精氨酸基因在大肠杆菌中的染色体定位以及携带它们的F'因子

CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F' INCORPORATING THEM.

作者信息

ISHIBASHI M, SUGINO Y, HIROTA Y

出版信息

J Bacteriol. 1964 Mar;87(3):554-61. doi: 10.1128/jb.87.3.554-561.1964.

DOI:10.1128/jb.87.3.554-561.1964
PMID:14127570
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC277053/
Abstract

Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is located on the chromosome, near Arg(2) (arginine) and between Sm (streptomycin) and Ade (adenine). A new F' (called F(15)) carrying the Thy (thymine) gene as well as the Arg(2) gene was discovered. The Thy(+) (thymine independent) gene on F(15) relieves the defect of all Thy(-) (thymine requiring) mutants of E. coli examined, such as E. coli 15 T(-), B3, and Thy(-) mutants obtained by aminopterine treatment. It can also be transferred to Thy(-) mutants of Salmonella typhimurium and Serratia marcescens, converting them to Thy(+).

摘要

石桥正秀（日本大阪大学，大阪）、杉野义信和广田幸德。大肠杆菌中胸腺嘧啶和精氨酸基因的染色体定位以及携带它们的F'因子。《细菌学杂志》87:554 - 561。1964年。——大肠杆菌K - 12中负责胸腺嘧啶需求或自主性的基因位于染色体上，靠近精氨酸（2）基因，在链霉素和腺嘌呤之间。发现了一种新的携带胸腺嘧啶（Thy）基因以及精氨酸（2）基因的F'因子（称为F（15））。F（15）上的胸腺嘧啶自主性（Thy（+））基因可弥补所检测的大肠杆菌所有胸腺嘧啶需求型（Thy（-））突变体的缺陷，如大肠杆菌15 T（-）、B3以及经氨甲蝶呤处理获得的Thy（-）突变体。它还能转移至鼠伤寒沙门氏菌和粘质沙雷氏菌的Thy（-）突变体，使其转变为Thy（+）。

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