PITTARD J, ADELBERG E A
J Bacteriol. 1963 Jun;85(6):1402-8. doi: 10.1128/jb.85.6.1402-1408.1963.
Pittard, James (Yale University, New Haven, Conn.) and Edward A. Adelberg. Gene transfer by F' strains of Escherichia coli K-12. II. Interaction between F-merogenote and chromosome during transfer. J. Bacteriol. 85:1402-1408. 1963.-When F' strains harboring the F-merogenate F(14) are mated with female recipients, the transfer of the F-merogenote begins, in the majority of cases, before chromosome transfer. The markers on F(14) are transferred in the sequence met-1, arg-1, ilva-16, and sex-factor, met-1 being transferred first and sex-factor being transferred last, 9 min after met-1. In the class of zygotes that have received both the F-merogenote marker met-1 and the chromosomal marker xyl or mal, the gradient of recombination frequencies for the F-merogenote markers arg-1 and ilva-16 is much steeper than in the corresponding zygotes that have not received chromosomal markers. In F' strains which exhibit an increased frequency of transfer of chromosome markers, this gradient of recombination frequencies for merogenote markers is much steeper. An analysis of experiments involving an F' strain with a much shorter F-merogenote, F(16), and of a triparental mating in which F-merogenote and chromosome were transferred from different donor cells reveals that the effect of chromosome transfer on the recovery of distal F-merogenote markers in the zygotes is not due to any form of postzygotic elimination. It is suggested that, when F' strains which are transferring F-merogenote begin to transfer chromosome, the latter event causes breakage of the F-merogenote. A second consequence of this interaction is a delay of 8 to 10 min in the first appearance of chromosomal markers in the zygotes.
皮塔德，詹姆斯（耶鲁大学，康涅狄格州纽黑文）和爱德华·A·阿德尔伯格。大肠杆菌K-12的F'菌株介导的基因转移。II. 转移过程中F-部分合子与染色体之间的相互作用。《细菌学杂志》85:1402 - 1408。1963年。 - 当携带F-部分合子F(14)的F'菌株与雌性受体交配时，在大多数情况下，F-部分合子的转移在染色体转移之前就开始了。F(14)上的标记按met-1、arg-1、ilvA-16和性因子的顺序转移，met-1最先转移，性因子在met-1转移9分钟后最后转移。在既接受了F-部分合子标记met-1又接受了染色体标记xyl或mal的合子类别中，F-部分合子标记arg-1和ilvA-16的重组频率梯度比未接受染色体标记的相应合子中的梯度陡得多。在染色体标记转移频率增加的F'菌株中，部分合子标记的这种重组频率梯度要陡得多。对涉及一个F-部分合子短得多的F'菌株F(16)的实验以及一个三方交配（其中F-部分合子和染色体从不同供体细胞转移）的分析表明，染色体转移对合子中远端F-部分合子标记回收的影响不是由于任何形式的合子后消除。有人提出，当正在转移F-部分合子的F'菌株开始转移染色体时，后一事件会导致F-部分合子断裂。这种相互作用的第二个结果是合子中染色体标记首次出现延迟8至10分钟。
