PITTARD J, LOUTIT J S, ADELBERG E A
J Bacteriol. 1963 Jun;85(6):1394-401. doi: 10.1128/jb.85.6.1394-1401.1963.
Pittard, James (Yale University, New Haven, Conn.), John S. Loutit, and Edward A. Adelberg. Gene transfer by F' strains of Escherichia coli K-12. I. Delay in initiation of chromosome transfer. J. Bacteriol. 85:1394-1401. 1963.-Two different F' strains were found to transfer both F-merogenote and chromosomal markers to recipient strains. When zygotes having received different chromosomal markers from the male were selected, a high percentage of them were found to have also received F-merogenote markers under conditions in which the mating of more than one male cell with a single female cell was excluded. An analysis of the kinetics of zygote formation revealed that chromosomal markers began to be transferred from F' strains 8 to 10 min later than from their analogous Hfr strains. The rate of chromosome transfer is the same from both F' and Hfr strains, and the difference observed in kinetic experiments was shown not to be due to any difference between the sequence of chromosomal genes in the F' and Hfr strains. F' strains and Hfr strains showed no difference in ability to form specific pairs with female recipients shortly after mixing. The observed delay in the transfer of chromosomal markers from F' cells thus means that, after specific pair formation, F' cells take 8 to 10 min longer than Hfr cells to initiate chromosome transfer.
皮塔德，詹姆斯（耶鲁大学，康涅狄格州纽黑文），约翰·S. 洛蒂特，以及爱德华·A. 阿德尔伯格。大肠杆菌K - 12 F' 菌株介导的基因转移。I. 染色体转移起始的延迟。《细菌学杂志》85:1394 - 1401。1963年。——发现两种不同的F' 菌株可将F - 部分合子和染色体标记物转移至受体菌株。当选择从雄性菌株获得不同染色体标记物的合子时，发现在排除一个以上雄性细胞与单个雌性细胞交配的条件下，其中很大一部分合子也获得了F - 部分合子标记物。对合子形成动力学的分析表明，染色体标记物从F' 菌株开始转移的时间比从其类似的高频重组（Hfr）菌株晚8至10分钟。F' 菌株和Hfr菌株的染色体转移速率相同，动力学实验中观察到的差异并非由于F' 菌株和Hfr菌株染色体基因序列的任何差异所致。F' 菌株和Hfr菌株在与雌性受体混合后不久形成特异性配对的能力上没有差异。因此，观察到的F' 细胞染色体标记物转移延迟意味着，在形成特异性配对后，F' 细胞比Hfr细胞多花8至10分钟来启动染色体转移。
