Allison D P, Warmack R J, Bottomley L A, Thundat T, Brown G M, Woychik R P, Schrick J J, Jacobson K B, Ferrell T L
Health and Safety Research Division, Oak Ridge National Laboratory, TN 37830-6123.
Ultramicroscopy. 1992 Jul;42-44 ( Pt B):1088-94. doi: 10.1016/0304-3991(92)90407-b.
pBS+ plasmid deoxyribonucleic acid (DNA) was imaged by scanning tunneling microscopy (STM) after mounting microdroplets by aerosol deposition onto heated epitaxial gold surfaces. However, the instability of the adsorbate to forces exerted by the tunneling tip points out the need for more aggressive bonding of molecules to surfaces. We describe a sensitive assay for the qualitative and quantitative evaluation of chemical agents to influence binding of DNA to surfaces using 32P-labeled pBS+ plasmid DNA. We propose that such an assay can make an important contribution to immobilization techniques prior to STM imaging.
通过气溶胶沉积将微滴安装到加热的外延金表面后，利用扫描隧道显微镜（STM）对pBS +质粒脱氧核糖核酸（DNA）进行成像。然而，吸附物对隧道尖端施加的力不稳定，这表明需要更积极地将分子与表面结合。我们描述了一种灵敏的检测方法，用于使用32P标记的pBS +质粒DNA对影响DNA与表面结合的化学试剂进行定性和定量评估。我们认为，这种检测方法可以在STM成像之前的固定技术中做出重要贡献。
