用于扫描探针显微镜的DNA固定化

Allison D P, Bottomley L A, Thundat T, Brown G M, Woychik R P, Schrick J J, Jacobson K B, Warmack R J

Health and Safety Research Division, Oak Ridge National Laboratory, TN 37831-6123.

Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10129-33. doi: 10.1073/pnas.89.21.10129.

Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented.

展示了化学结合到表面的未包被质粒DNA完整分子的可重复扫描隧道显微镜和原子力显微镜图像。DNA通过化学介导固定到表面以及随后对DNA分子进行扫描隧道显微镜成像表明，扫描探针显微镜固有的探针尖端施加力导致分子不稳定的问题是可以避免的。